Secretory Vesicle Clustering in Fungal Filamentous Cells Does Not Require Directional Growth.
Cdc42
fungi
membrane traffic
morphology
optogenetics
polarity
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
20 08 2019
20 08 2019
Historique:
received:
30
12
2018
revised:
22
04
2019
accepted:
18
07
2019
entrez:
22
8
2019
pubmed:
23
8
2019
medline:
1
9
2020
Statut:
ppublish
Résumé
During symmetry breaking, the highly conserved Rho GTPase Cdc42 becomes stabilized at a defined site via an amplification process. However, little is known about how a new polarity site is established in an already asymmetric cell-a critical process in a changing environment. The human fungal pathogen Candida albicans switches from budding to filamentous growth in response to external cues, a transition controlled by Cdc42. Here, we have used optogenetic manipulation of cell polarity to reset growth in asymmetric filamentous C. albicans cells. We show that increasing the level of active Cdc42 on the plasma membrane results in disruption of the exocyst subunit Sec3 localization and a striking de novo clustering of secretory vesicles. This new cluster of secretory vesicles is highly dynamic, moving by hops and jumps, until a new growth site is established. Our results reveal that secretory vesicle clustering can occur in the absence of directional growth.
Identifiants
pubmed: 31433995
pii: S2211-1247(19)30966-0
doi: 10.1016/j.celrep.2019.07.062
pii:
doi:
Substances chimiques
Fungal Proteins
0
Guanosine Triphosphate
86-01-1
cdc42 GTP-Binding Protein
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2231-2245.e5Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.