Cryptosporidium Genotyping for Epidemiology Tracking.

Animals Cryptosporidiosis / epidemiology Cryptosporidium / genetics Cryptosporidium parvum / genetics DNA, Protozoan / genetics Feces / parasitology Genotype Genotyping Techniques / methods Humans Polymerase Chain Reaction / methods RNA, Ribosomal / genetics Sequence Analysis, DNA / instrumentation Workflow
DNA sequencing Genotyping Molecular epidemiology PCR PCR-RFLP Subtyping

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 28 8 2019
pubmed: 28 8 2019
medline: 24 11 2020
Statut: ppublish

Résumé

Cryptosporidium genotyping has made significant contributions to the species structure and population genetics of Cryptosporidium spp. In addition, the standardized method has shown utility in epidemiologic investigations identifying case linkages and contamination sources that could not be determined with traditional epidemiologic tools. The standardized method for Cryptosporidium genotyping from stool specimens described here comprises multiple individual protocols to amplify and sequence regions of the SSU rRNA and gp60 genes of Cryptosporidium.

Identifiants

DOI: 10.1007/978-1-4939-9748-0_7 PMID: 31452159
pubmed: 31452159
doi: 10.1007/978-1-4939-9748-0_7
doi:

Substances chimiques

DNA, Protozoan 0
RNA, Ribosomal 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

103-116

Auteurs

Dawn M Roellig (DM)

Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. DMRoellig@cdc.gov.

Lihua Xiao (L)

Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Maladies de l'animal › Parasitoses animales › Protozooses animales › Cryptosporidiose › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Eucaryotes › Alveolata › Apicomplexa › Coccidia › Eimeriida › Cryptosporidiidae › Cryptosporidium › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Eucaryotes › Alveolata › Apicomplexa › Coccidia › Eimeriida › Cryptosporidiidae › Cryptosporidium › Cryptosporidium parvum › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN des protozoaires › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Liquides et sécrétions biologiques › Fèces › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Phénomènes génétiques › Génotype › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de génotypage › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ADN › Cryptosporidium Genotyping for Epidemiology Tracking.
questionsmedicales.fr › Sciences de l'information › Analyse des systèmes › Flux de travaux › Cryptosporidium Genotyping for Epidemiology Tracking.