Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins.
ADAMTS Proteins
/ genetics
Cell Adhesion Molecules, Neuronal
/ genetics
Extracellular Matrix Proteins
/ genetics
Gene Expression
HEK293 Cells
Humans
Nerve Tissue Proteins
/ genetics
Procollagen N-Endopeptidase
/ genetics
Protein Engineering
Recombinant Proteins
/ metabolism
Reelin Protein
Serine Endopeptidases
/ genetics
Expi293
HEK293T
Lipofection
SDS-PAGE
Western blotting
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
30
8
2019
pubmed:
30
8
2019
medline:
11
4
2020
Statut:
ppublish
Résumé
Reelin is a large secreted protein that is essential for the brain development and function. Reelin is negatively regulated by the specific cleavage by a disintegrin and metalloproteinase with thrombospondin type 1 motifs 3 (ADAMTS-3) which is also secreted from neurons. It is likely that there are other proteases that can cleave Reelin. This chapter describes the protocol for expression and handling of recombinant Reelin and ADAMTS-3 proteins to facilitate investigation of these proteins.
Identifiants
pubmed: 31463905
doi: 10.1007/978-1-4939-9698-8_8
doi:
Substances chimiques
Cell Adhesion Molecules, Neuronal
0
Extracellular Matrix Proteins
0
Nerve Tissue Proteins
0
Recombinant Proteins
0
Reelin Protein
0
RELN protein, human
EC 3.4.21.-
Serine Endopeptidases
EC 3.4.21.-
ADAMTS Proteins
EC 3.4.24.-
ADAMTS3 protein, human
EC 3.4.24.-
Procollagen N-Endopeptidase
EC 3.4.24.14
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM