Proteins required for vacuolar function are targets of lysine polyphosphorylation in yeast.
Adaptor Protein Complex 3
Adaptor Proteins, Vesicular Transport
/ metabolism
Endopeptidases
/ metabolism
Lysine
/ metabolism
Phosphorylation
Protein Binding
Protein Interaction Maps
Protein Processing, Post-Translational
Proteome
/ metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
/ metabolism
Vacuoles
/ metabolism
Apl5
Prb1
polyP
polyphosphate
polyphosphorylation
vacuole
yeast
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
12
07
2019
revised:
22
08
2019
accepted:
23
08
2019
pubmed:
30
8
2019
medline:
4
8
2020
entrez:
30
8
2019
Statut:
ppublish
Résumé
Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of polyP. In this study, we evaluated the polyphosphorylation status of over 200 candidate targets in Saccharomyces cerevisiae. We report eight new polyphosphorylated proteins that interact genetically and physically with previous targets implicated in ribosome biogenesis. The expanded target network includes vacuolar proteins Prb1 and Apl5, whose modification with polyP suggests a model for feedback regulation of polyP synthesis, while raising questions regarding the location of polyphosphorylation in vivo.
Identifiants
pubmed: 31466120
doi: 10.1002/1873-3468.13588
doi:
Substances chimiques
APL5 protein, S cerevisiae
0
Adaptor Protein Complex 3
0
Adaptor Proteins, Vesicular Transport
0
Proteome
0
Saccharomyces cerevisiae Proteins
0
Endopeptidases
EC 3.4.-
PRB1 protein, S cerevisiae
EC 3.4.-
Lysine
K3Z4F929H6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21-30Subventions
Organisme : CIHR
ID : PJT-148722
Pays : Canada
Informations de copyright
© 2019 Federation of European Biochemical Societies.
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