The Validation of Neogen Reveal® Q+ for DON.

Edible Grain / chemistry Food Contamination / analysis Hordeum Mycotoxins / analysis Trichothecenes / analysis Triticum

Journal

Journal of AOAC International
ISSN: 1944-7922
Titre abrégé: J AOAC Int
Pays: England
ID NLM: 9215446

Informations de publication

Date de publication:
01 Jan 2020
Historique:
received: 02 08 2019
pubmed: 31 8 2019
medline: 29 6 2021
entrez: 31 8 2019
Statut: ppublish

Résumé

Reveal® Q+ for DON is an immunochromatographic test for quantitative determination of deoxynivalenol (DON) in grains. A study was conducted to validate performance of this method for determination of DON in naturally contaminated corn and wheat and in DON-spiked corn/soy blend, soybeans, barley, malted barley, buckwheat, brown rice, sorghum, and distillers dried grain. In addition to matrix testing, LOD, linearity, selectivity, robustness, and stability/lot-to-lot consistency testing were performed. The LOD was determined to be 0.014 ppm in corn and 0.037 ppm in wheat, and the LOQ 0.042 ppm in corn and 0.11 ppm in wheat. Recovery ranged from 90 to 104% across a range of reference values of 0.5 to 34.5 ppm. Linearity calculation comparing test results with reference values produced R2 values of 0.999 in both matrixes. Internal results with corn and wheat were corroborated in independent laboratory testing. For DON-spiked commodities, mean recovery across a range of DON concentration from 0.5 to 30 ppm ranged from 90 to 109%. Results of selectivity testing showed no cross-reactivity with other mycotoxins and no interference in detection of DON. Reagent lot-to-lot consistency and stability studies showed consistent results across a range of DON levels and established expiration dating for the test of 18 months after manufacture when stored under specified conditions. Conclusions and Highlights: The Reveal Q+ for DON test offers reliable performance as well as the advantages of aqueous sample extraction, procedural simplicity, minimal labor and equipment requirements, and rapid results. The Reveal Q+ for DON test is validated as a Performace Tested Method in Corn, Wheat, and a variety of other grains. The test provides rapid results from a simple aqueous extraction and requires minimal labor and equipment.

Sections du résumé

BACKGROUND BACKGROUND
Reveal® Q+ for DON is an immunochromatographic test for quantitative determination of deoxynivalenol (DON) in grains.
OBJECTIVE OBJECTIVE
A study was conducted to validate performance of this method for determination of DON in naturally contaminated corn and wheat and in DON-spiked corn/soy blend, soybeans, barley, malted barley, buckwheat, brown rice, sorghum, and distillers dried grain.
METHODS METHODS
In addition to matrix testing, LOD, linearity, selectivity, robustness, and stability/lot-to-lot consistency testing were performed.
RESULTS RESULTS
The LOD was determined to be 0.014 ppm in corn and 0.037 ppm in wheat, and the LOQ 0.042 ppm in corn and 0.11 ppm in wheat. Recovery ranged from 90 to 104% across a range of reference values of 0.5 to 34.5 ppm. Linearity calculation comparing test results with reference values produced R2 values of 0.999 in both matrixes. Internal results with corn and wheat were corroborated in independent laboratory testing. For DON-spiked commodities, mean recovery across a range of DON concentration from 0.5 to 30 ppm ranged from 90 to 109%. Results of selectivity testing showed no cross-reactivity with other mycotoxins and no interference in detection of DON. Reagent lot-to-lot consistency and stability studies showed consistent results across a range of DON levels and established expiration dating for the test of 18 months after manufacture when stored under specified conditions. Conclusions and Highlights: The Reveal Q+ for DON test offers reliable performance as well as the advantages of aqueous sample extraction, procedural simplicity, minimal labor and equipment requirements, and rapid results.
CONCLUSIONS CONCLUSIONS
The Reveal Q+ for DON test is validated as a Performace Tested Method in Corn, Wheat, and a variety of other grains.
HIGHLIGHTS CONCLUSIONS
The test provides rapid results from a simple aqueous extraction and requires minimal labor and equipment.

Identifiants

DOI: 10.5740/jaoacint.19-0252 PMID: 31466556
pubmed: 31466556
pii: 5717547
doi: 10.5740/jaoacint.19-0252
doi:

Substances chimiques

Mycotoxins 0
Trichothecenes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

176-183

Informations de copyright

© AOAC INTERNATIONAL 2020.

Auteurs

R Lucas Gray (RL)

Neogen Corporation, 620 Lesher Pl, Lansing, MI 48912.

Alex Kostin (A)

Neogen Corporation, 620 Lesher Pl, Lansing, MI 48912.

Gabrielle Anderson (G)

Neogen Corporation, 620 Lesher Pl, Lansing, MI 48912.

Brooke Roman (B)

Neogen Corporation, 620 Lesher Pl, Lansing, MI 48912.

Frank Klein (F)

Neogen Corporation, 620 Lesher Pl, Lansing, MI 48912.

Robert Donofrio (R)

Neogen Corporation, 620 Lesher Pl, Lansing, MI 48912.

Tetsuhito Goto (T)

Central Science Commerce, Inc., Ushiku, Japan.

Mary Trucksess (M)

U.S. Department of Health and Human Services, Washington, DC.

Wayne Ziemer (W)

Consultant, Loganville, GA.

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Classifications MeSH

questionsmedicales.fr › Aliments et boissons › Aliments › Graines › Grains comestibles › The Validation of Neogen Reveal® Q+ for DON.
questionsmedicales.fr › Environnement et santé publique › Santé publique › Qualité alimentaire › Sécurité des aliments › Contamination des aliments › The Validation of Neogen Reveal® Q+ for DON.
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Hordeum › The Validation of Neogen Reveal® Q+ for DON.
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Mycotoxines › The Validation of Neogen Reveal® Q+ for DON.
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Mycotoxines › Trichothécènes › The Validation of Neogen Reveal® Q+ for DON.
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Triticum › The Validation of Neogen Reveal® Q+ for DON.