Analysis of the 56-kDa type specific antigen gene of Orientia tsutsugamushi from northern Vietnam.
Antigens, Bacterial
/ chemistry
Bacterial Proteins
/ chemistry
Genotype
High-Throughput Nucleotide Sequencing
Humans
Molecular Typing
Molecular Weight
Orientia tsutsugamushi
/ classification
Phylogeny
Phylogeography
Prevalence
Scrub Typhus
/ diagnosis
Sequence Analysis, DNA
Severity of Illness Index
Vietnam
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
03
04
2019
accepted:
09
08
2019
entrez:
31
8
2019
pubmed:
31
8
2019
medline:
12
3
2020
Statut:
epublish
Résumé
Scrub typhus has been documented since 1932 in Vietnam, however, the disease burden of scrub typhus remains poorly understood in the country. We conducted this study to describe the phylogenetic analysis of the 56-kDa type-specific antigen (TSA) gene of Orientia tsutsugamushi associated with PCR positive cases of scrub typhus. Of 116 positive samples, 65 type-specific antigen gene sequences were obtained and classified into 3 genogroups: Karp, Kato and Gilliam. The Karp genogroup was the most frequently detected phylogenetic cluster in the study with 30 samples (46%), followed by Kato and Gilliam with 20 (31%) and 15 (23%), respectively. All sequences showed 94-100% nucleotide similarity to reference sequences collected in the central part of Vietnam in 2017. Patients infected with Karp genogroup were more likely to have significant thrombocytopenia than the other genogroups. These results suggest that any scrub typhus vaccine considered for use in Vietnam should provide protection against each of these 3 genogroups.
Identifiants
pubmed: 31469878
doi: 10.1371/journal.pone.0221588
pii: PONE-D-19-09529
pmc: PMC6716651
doi:
Substances chimiques
Antigens, Bacterial
0
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0221588Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 106680/Z/14/Z
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Microbiol Immunol. 2001;45(6):439-46
pubmed: 11497219
Bull Soc Pathol Exot Filiales. 1964 Mar-Apr;57:257-63
pubmed: 14184777
Am J Trop Med Hyg. 2004 Apr;70(4):351-6
pubmed: 15100446
Clin Microbiol Infect. 2008 Feb;14(2):168-73
pubmed: 18076670
FEMS Immunol Med Microbiol. 2008 Apr;52(3):335-42
pubmed: 18312580
Clin Infect Dis. 2009 Mar 15;48 Suppl 3:S203-30
pubmed: 19220144
Am J Trop Med Hyg. 2010 Sep;83(3):658-63
pubmed: 20810835
J Clin Microbiol. 1990 Sep;28(9):2111-3
pubmed: 2121793
Infect Genet Evol. 2013 Apr;15:35-42
pubmed: 21241829
Am J Trop Med Hyg. 2011 Nov;85(5):924-30
pubmed: 22049051
PLoS One. 2012;7(9):e44269
pubmed: 22970193
PLoS One. 2012;7(10):e46997
pubmed: 23071693
J Formos Med Assoc. 2013 Apr;112(4):201-7
pubmed: 23537866
Am J Trop Med Hyg. 2015 May;92(5):972-978
pubmed: 25778504
PLoS One. 2015 May 29;10(5):e0114930
pubmed: 26024375
Emerg Infect Dis. 2016 Nov;22(11):1992-1995
pubmed: 27767909
Vector Borne Zoonotic Dis. 2017 Apr;17(4):260-267
pubmed: 28055333
PLoS Negl Trop Dis. 2017 Feb 27;11(2):e0005397
pubmed: 28241043
Am J Trop Med Hyg. 2017 May;96(5):1084-1087
pubmed: 28500808
Trans R Soc Trop Med Hyg. 2017 Mar 1;111(3):137-139
pubmed: 28531311
Crit Care Med. 1985 Oct;13(10):818-29
pubmed: 3928249
Microbiol Immunol. 1996;40(10):743-7
pubmed: 8981347