Current advances in engineering tools for Pichia pastoris.

Gene Editing Pichia Promoter Regions, Genetic Recombinant Proteins Synthetic Biology

Journal

Current opinion in biotechnology
ISSN: 1879-0429
Titre abrégé: Curr Opin Biotechnol
Pays: England
ID NLM: 9100492

Informations de publication

Date de publication:
10 2019
Historique:
received: 21 01 2019
revised: 02 06 2019
accepted: 18 06 2019
pubmed: 31 8 2019
medline: 8 2 2020
entrez: 31 8 2019
Statut: ppublish

Résumé

In the past two to five years innovative DNA tools and inventive methodologies accelerated the speed of engineering of Komagataella phaffii (Pichia pastoris) for the efficient expression of intracellular and secreted proteins. Going beyond the standard approaches employing single heterologous genes or simultaneous expression of several different genes under the control of identical promoter and terminator sequences, balanced and consecutive co-expression of multiple genes (a, b) combined with simple host genome editing (c) now opens new opportunities for manufacturing of recombinant proteins or chemicals made by whole cell biocatalysis or synthetic biology.

Identifiants

DOI: 10.1016/j.copbio.2019.06.002 PMID: 31470258
pubmed: 31470258
pii: S0958-1669(18)30193-9
doi: 10.1016/j.copbio.2019.06.002
pii:
doi:

Substances chimiques

Recombinant Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

175-181

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Jasmin E Fischer (JE)

Bisy e.U., Hofstätten/Raab, Austria; Institute of Molecular Biotechnology, NAWI Graz University of Technology, Graz, Austria.

Anton Glieder (A)

Bisy e.U., Hofstätten/Raab, Austria; Institute of Molecular Biotechnology, NAWI Graz University of Technology, Graz, Austria. Electronic address: anton.glieder@tugraz.at.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Current advances in engineering tools for...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Pichia › Current advances in engineering tools for...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › Current advances in engineering tools for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Current advances in engineering tools for...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Biologie synthétique › Current advances in engineering tools for...