Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut.

Adaptor Proteins, Signal Transducing / metabolism Adaptor Proteins, Vesicular Transport / metabolism Animals Apoptosis Regulatory Proteins / metabolism Dietary Proteins / metabolism Disease Models, Animal Enterocytes / metabolism Female Gastrointestinal Microbiome Gene Deletion Gene Expression Regulation, Developmental Ileum / embryology Intestinal Absorption Intestines / embryology Kwashiorkor / metabolism Ligands Lysosomes / metabolism Male Membrane Proteins / metabolism Mice Receptors, Cell Surface / metabolism Zebrafish Zebrafish Proteins / metabolism
Cubilin Dab2 inter-organ transport intestine kwashiorkor lysosome-rich enterocytes (LREs) malnutrition mouse protein absorption zebrafish

Journal

Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028

Informations de publication

Date de publication:
07 10 2019
Historique:
received: 31 03 2019
revised: 06 06 2019
accepted: 29 07 2019
pubmed: 3 9 2019
medline: 28 5 2020
entrez: 3 9 2019
Statut: ppublish

Résumé

The guts of neonatal mammals and stomachless fish have a limited capacity for luminal protein digestion, which allows oral acquisition of antibodies and antigens. However, how dietary protein is absorbed during critical developmental stages when the gut is still immature is unknown. Here, we show that specialized intestinal cells, which we call lysosome-rich enterocytes (LREs), internalize dietary protein via receptor-mediated and fluid-phase endocytosis for intracellular digestion and trans-cellular transport. In LREs, we identify a conserved endocytic machinery, composed of the scavenger receptor complex Cubilin/Amnionless and Dab2, that is required for protein uptake by LREs and for growth and survival of larval zebrafish. Moreover, impairing LRE function in suckling mice, via conditional deletion of Dab2, leads to stunted growth and severe protein malnutrition reminiscent of kwashiorkor, a devastating human malnutrition syndrome. These findings identify digestive functions and conserved molecular mechanisms in LREs that are crucial for vertebrate growth and survival.

Identifiants

DOI: 10.1016/j.devcel.2019.08.001 PMID: 31474562 PMC: PMC6783362
pubmed: 31474562
pii: S1534-5807(19)30661-6
doi: 10.1016/j.devcel.2019.08.001
pmc: PMC6783362
mid: NIHMS1537093
pii:
doi:

Substances chimiques

Adaptor Proteins, Signal Transducing 0
Adaptor Proteins, Vesicular Transport 0
Amn protein, mouse 0
Apoptosis Regulatory Proteins 0
Dab2 protein, mouse 0
Dab2 protein, zebrafish 0
Dietary Proteins 0
Ligands 0
Membrane Proteins 0
Receptors, Cell Surface 0
Zebrafish Proteins 0
intrinsic factor-cobalamin receptor 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7-20.e6

Subventions

Organisme : NIDDK NIH HHS
ID : P01 DK094779
Pays : United States
Organisme : NIDDK NIH HHS
ID : F31 DK111137
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK081426
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK117981
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK113123
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR055926
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007568
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR067203
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK121007
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

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Auteurs

Jieun Park (J)

Department of Cell Biology, Duke University, Durham, NC 27710, USA; Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA.

Daniel S Levic (DS)

Department of Cell Biology, Duke University, Durham, NC 27710, USA.

Kaelyn D Sumigray (KD)

Department of Dermatology, Duke University Medical Center, Durham, NC 27710, USA.

Jennifer Bagwell (J)

Department of Cell Biology, Duke University, Durham, NC 27710, USA.

Oznur Eroglu (O)

Department of Cell Biology, Duke University, Durham, NC 27710, USA.

Carina L Block (CL)

Department of Psychology and Neuroscience, Duke University, Durham, NC 27710, USA.

Cagla Eroglu (C)

Department of Cell Biology, Duke University, Durham, NC 27710, USA; Department of Neurobiology, Duke University, Durham, NC 27710, United States; Regeneration Next Initiative, Duke University, Durham, NC 27710, United States.

Robert Barry (R)

Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Colin R Lickwar (CR)

Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.

John F Rawls (JF)

Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.

Stephen A Watts (SA)

Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Terry Lechler (T)

Department of Cell Biology, Duke University, Durham, NC 27710, USA; Department of Dermatology, Duke University Medical Center, Durham, NC 27710, USA.

Michel Bagnat (M)

Department of Cell Biology, Duke University, Durham, NC 27710, USA; Regeneration Next Initiative, Duke University, Durham, NC 27710, United States. Electronic address: michel.bagnat@duke.edu.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines adaptatrices de la transduction du signal › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines du transport vésiculaire › Protéines adaptatrices du transport vésiculaire › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Eucaryotes › Animaux › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Aliments et boissons › Aliments › Protéines alimentaires › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Cellules › Cellules épithéliales › Entérocytes › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbiome gastro-intestinal › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Délétion de séquence › Délétion de gène › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes au cours du développement › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Système digestif › Tube digestif › Tube digestif inférieur › Iléum › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Phénomènes physiologiques de l'appareil buccodentaire et de l'appareil digestif › Phénomènes physiologiques de l'appareil digestif › Absorption gastro-intestinale › Absorption intestinale › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Système digestif › Tube digestif › Intestins › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Troubles nutritionnels › Malnutrition › Malnutrition aigüe sévère › Kwashiorkor › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Ligands › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Lysosomes › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › Lysosome-Rich Enterocytes Mediate Protein...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de poisson › Protéines de poisson-zèbre › Lysosome-Rich Enterocytes Mediate Protein...