A comparative analysis of Smad-responsive motifs identifies multiple regulatory inputs for TGF-β transcriptional activation.
ChIP-sequencing (ChIP-seq)
DNA-binding protein
SMAD transcription factor
signal transduction
transcription factor
transcription regulation
transforming growth factor β (TGF-β)
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
18 10 2019
18 10 2019
Historique:
received:
21
06
2019
revised:
29
08
2019
pubmed:
5
9
2019
medline:
23
6
2020
entrez:
5
9
2019
Statut:
ppublish
Résumé
Smad proteins are transcriptional regulators activated by TGF-β. They are known to bind to two distinct Smad-responsive motifs, namely the Smad-binding element (SBE) (5'-GTCTAGAC-3') and CAGA motifs (5'-AGCCAGACA-3' or 5'-TGTCTGGCT-3'). However, the mechanisms by which these motifs promote Smad activity are not fully elucidated. In this study, we performed DNA CASTing, binding assays, ChIP sequencing, and quantitative RT-PCR to dissect the details of Smad binding and function of the SBE and CAGA motifs. We observed a preference for Smad3 to bind CAGA motifs and Smad4 to bind SBE, and that either one SBE or a triple-CAGA motif forms a
Identifiants
pubmed: 31481467
pii: S0021-9258(20)33787-X
doi: 10.1074/jbc.RA119.009877
pmc: PMC6802517
doi:
Substances chimiques
SMAD2 protein, human
0
SMAD3 protein, human
0
SMAD4 protein, human
0
Smad2 Protein
0
Smad3 Protein
0
Smad4 Protein
0
Transforming Growth Factor beta
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15466-15479Informations de copyright
© 2019 Itoh et al.
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