Molecular characterization of Equine Infectious Anemia Viruses using targeted sequence enrichment and next generation sequencing.
Equine infectious anemia
Horse
NGS
Phylogeny
Retrovirus
Targeted enrichment system
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
03
05
2019
revised:
13
08
2019
accepted:
16
08
2019
pubmed:
8
9
2019
medline:
12
6
2020
entrez:
8
9
2019
Statut:
ppublish
Résumé
Equine infectious anemia virus (EIAV) is responsible of acute disease episodes characterized by fever, anemia, thrombocytopenia and anorexia in equids. The high mutation rate in EIAV genome limited the number of full genome sequences availability. In the present study, we used the SureSelect target enrichment system with Illumina Next Generation Sequencing to characterize the proviral DNA of Equine Infectious Anemia Virus (EIAV) from asymptomatic horses. This approach allows a direct sequencing of the EIAV whole genome without cloning or amplification steps and we could obtain for the first time the complete genomic DNA sequences of French EIAV strains. We analyzed their phylogenetic relationship and genetic variability by comparison with 17 whole EIAV genome sequences from different parts of the world. The results obtained provide new insights into the molecular detection of EIAV and genetic diversity of European viral strains.
Identifiants
pubmed: 31493650
pii: S0042-6822(19)30229-6
doi: 10.1016/j.virol.2019.08.016
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
121-129Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.