Proof of Concept To Achieve Infinite Selectivity for the Chromatographic Separation of Therapeutic Proteins.

Antibodies, Monoclonal / isolation & purification Chromatography, Reverse-Phase / methods Humans Immunoconjugates / isolation & purification Mass Spectrometry / methods Proof of Concept Study

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
15 10 2019
Historique:
pubmed: 14 9 2019
medline: 29 9 2020
entrez: 14 9 2019
Statut: ppublish

Résumé

Reversed phase liquid chromatography (RPLC) is a widely used technique for the analytical characterization of proteins biopharmaceuticals, due to its inherent compatibility with mass spectrometry (MS). However, this chromatographic mode suffers from limited selectivity when analyzing large molecules. Due to the on/off mechanism observed with large solutes in RPLC (

Identifiants

DOI: 10.1021/acs.analchem.9b03005 PMID: 31514494
pubmed: 31514494
doi: 10.1021/acs.analchem.9b03005
doi:

Substances chimiques

Antibodies, Monoclonal 0
Immunoconjugates 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

12954-12961

Auteurs

Szabolcs Fekete (S)

School of Pharmaceutical Sciences , University of Geneva, University of Lausanne, CMU , Rue Michel-Servet, 1 , 1206 Geneva , Switzerland.
ORCID: 0000-0002-7357-0691

Alain Beck (A)

Center of Immunology Pierre Fabre , 5 Avenue Napoléon III , BP 60497, 74160 Saint-Julien-en-Genevois , France.

Jean-Luc Veuthey (JL)

School of Pharmaceutical Sciences , University of Geneva, University of Lausanne, CMU , Rue Michel-Servet, 1 , 1206 Geneva , Switzerland.

Davy Guillarme (D)

School of Pharmaceutical Sciences , University of Geneva, University of Lausanne, CMU , Rue Michel-Servet, 1 , 1206 Geneva , Switzerland.
ORCID: 0000-0001-7883-5823

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Proof of Concept To Achieve Infinite...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase inverse › Proof of Concept To Achieve Infinite...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Proof of Concept To Achieve Infinite...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Effets physiologiques des médicaments › Facteurs immunologiques › Immunoconjugués › Proof of Concept To Achieve Infinite...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Proof of Concept To Achieve Infinite...
questionsmedicales.fr › Disciplines des sciences naturelles › Science › Recherche › Étude de validation de principe › Proof of Concept To Achieve Infinite...