Serotonin is essential for eye regeneration in planaria Schmidtea mediterranea.
LC-MS
eye regeneration
phenylalanine hydroxylase
planaria
serotonin
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
22
07
2019
revised:
23
08
2019
accepted:
06
09
2019
pubmed:
19
9
2019
medline:
23
6
2020
entrez:
19
9
2019
Statut:
ppublish
Résumé
Planaria is an ideal system to study factors involved in regeneration and tissue homeostasis. Little is known about the role of metabolites and small molecules in stem cell maintenance and lineage specification in planarians. Using liquid chromatography and mass spectrometry (LC-MS)-based quantitative metabolomics, we determined the relative levels of metabolites in stem cells, progenitors, and differentiated cells of the planarian Schmidtea mediterranea. Tryptophan and its metabolic product serotonin are significantly enriched in stem cells and progenitor population. Serotonin biosynthesis in these cells is brought about by a noncanonical enzyme, phenylalanine hydroxylase. Knockdown of Smed-pah leads to complete disappearance of eyes in regenerating planaria, while exogenous supply of serotonin and its precursor rescues the eyeless phenotype. Our results demonstrate a key role for serotonin in eye regeneration.
Identifiants
pubmed: 31529697
doi: 10.1002/1873-3468.13607
doi:
Substances chimiques
Serotonin
333DO1RDJY
Tryptophan
8DUH1N11BX
Phenylalanine Hydroxylase
EC 1.14.16.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3198-3209Subventions
Organisme : DST Swarnajayanti Fellowship
ID : DST/SJF/LSA-02/2015-16
Pays : International
Organisme : Institute for Stem Cell Science and Regenerative Medicine
Pays : International
Informations de copyright
© 2019 Federation of European Biochemical Societies.
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