Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite.

Animals DNA / genetics High-Throughput Nucleotide Sequencing Molecular Weight Organic Chemicals / chemistry Thoracica / genetics
Amphibalanus amphitrite Barnacle Crustacean DNA extraction Next generation sequencing

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
01 12 2019
Historique:
received: 29 05 2019
revised: 10 09 2019
accepted: 15 09 2019
pubmed: 21 9 2019
medline: 11 8 2020
entrez: 21 9 2019
Statut: ppublish

Résumé

Next generation sequencing (NGS) technologies can provide an understanding of the molecular processes involved in marine fouling by Amphibalanus spp. barnacles. Here, seven methods for extracting DNA from A. amphitrite prosomata were assessed with respect to recovery, purity and size distribution. Methods incorporating organic extractions generally resulted in low recovery of fragmented DNA. The most promising method was the commercial E.Z.N.A. Blood DNA Mini kit, which provided tens of micrograms of DNA of sufficient molecular weight for use in long-read NGS library preparation. Other kits resulted in DNA preps suitable for short read length NGS platforms.

Identifiants

DOI: 10.1016/j.ab.2019.113441 PMID: 31539523
pubmed: 31539523
pii: S0003-2697(19)30510-X
doi: 10.1016/j.ab.2019.113441
pii:
doi:

Substances chimiques

Organic Chemicals 0
DNA 9007-49-2

Types de publication

Comparative Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

113441

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Janna N Schultzhaus (JN)

National Research Council Research Associateship Program, Washington, DC, 20001, USA.

Chris R Taitt (CR)

Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375, USA.

Beatriz Orihuela (B)

Duke University Marine Laboratory, Beaufort, NC, 28516, USA.

Madeline Smerchansky (M)

Naval Research Enterprise Internship Program, American Society for Engineering Education, 1818 N St. NW, Washington, DC, 20036, USA.

Zachary S Schultzhaus (ZS)

National Research Council Research Associateship Program, Washington, DC, 20001, USA.

Daniel Rittschof (D)

Duke University Marine Laboratory, Beaufort, NC, 28516, USA.

Kathryn J Wahl (KJ)

Chemistry Division, Naval Research Laboratory, Washington, DC, 20375, USA.

Christopher M Spillmann (CM)

Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375, USA. Electronic address: christopher.spillmann@nrl.navy.mil.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Comparison of seven methods for DNA extraction...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Comparison of seven methods for DNA extraction...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Séquençage nucléotidique à haut débit › Comparison of seven methods for DNA extraction...
questionsmedicales.fr › Phénomènes chimiques › Masse moléculaire › Comparison of seven methods for DNA extraction...
questionsmedicales.fr › Composés chimiques organiques › Comparison of seven methods for DNA extraction...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Crustacea › Thoracica › Comparison of seven methods for DNA extraction...