Understanding the role of SilE in the production of metal nanoparticles by Morganella psychrotolerans using MicroScale Thermophoresis.
Binding affinity
Metal nanoparticles
MicroScale Thermophoresis
SilE
Journal
New biotechnology
ISSN: 1876-4347
Titre abrégé: N Biotechnol
Pays: Netherlands
ID NLM: 101465345
Informations de publication
Date de publication:
25 Mar 2020
25 Mar 2020
Historique:
received:
23
11
2018
revised:
06
09
2019
accepted:
14
09
2019
pubmed:
21
9
2019
medline:
5
6
2020
entrez:
21
9
2019
Statut:
ppublish
Résumé
Metal nanoparticle synthesis has been observed in several species of bacteria but the underlying mechanisms of synthesis are not well understood. Morganella psychrotolerans is a Gram-negative psychrophilic bacterium that is able to tolerate relatively high concentrations of Cu and Ag ions, and it is through the associated resistance pathways that this species is able to convert metal ions to nanoparticles. The purpose of this study was to investigate the mechanism of nanoparticle synthesis, looking at the interaction of the metal binding protein SilE with metal ions using MicroScale Thermophoresis (MST). MST assays give a rapid and accurate determination of binding affinities, allowing for the testing of SilE with a range of environmentally significant metal ions. The binding affinities (K
Identifiants
pubmed: 31539639
pii: S1871-6784(18)31934-4
doi: 10.1016/j.nbt.2019.09.002
pii:
doi:
Substances chimiques
Metals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-4Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N002520/1
Pays : United Kingdom
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.