In vitro evaluation of the effects of Ultrasound Tongue Scraper on bacteria and biofilm formation.
Ultrasound Tongue Scraper
biofilm
microscopic analysis
oral malodor
red complex bacteria
Journal
Journal of investigative and clinical dentistry
ISSN: 2041-1626
Titre abrégé: J Investig Clin Dent
Pays: Australia
ID NLM: 101524471
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
22
02
2019
revised:
08
06
2019
accepted:
20
08
2019
pubmed:
22
9
2019
medline:
27
11
2019
entrez:
22
9
2019
Statut:
ppublish
Résumé
Oral malodor is a common condition caused by some Gram-negative oral bacteria, among which are the 3 red complex bacteria (RCB). The present study investigated the effectiveness of the Ultrasound Tongue Scraper (UTS) to disrupt the structural morphology of the bacteria and their biofilm. While developing over 72 hours, multispecies biofilms of RCB (Porphromonas gingivalis, Tryponema denticola, Tannerella forsythia) were treated every 24 hours with 1.6-MHz ultrasound waves generated with UTS. An untreated group served as controls. Confocal laser scanning microscopy was used to determine the biofilm thickness, biomass and live : dead cell ratio at each time point (24, 48 and 72 hours). Biofilm morphology and bacteria ultrastructure were viewed using scanning/transmission electron microscopy, respectively. Data were analyzed using ANOVA and Tukey tests. At each time point, the 3 variables were significantly lower in treated samples than the untreated. Significant biofilm disruption was observed in treated samples at each time period while the untreated had intact biofilm morphology. Cells in treated samples showed disrupted cell wall, cytoplasmic material, huge vacuoles and heterogeneity in electron density, while these cell organelles remained intact in untreated samples. The UTS has an inhibitory effect on RCB and could be useful for oral malodor management.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12471Subventions
Organisme : Starmoon
Informations de copyright
© 2019 John Wiley & Sons Australia, Ltd.
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