NET4 Modulates the Compactness of Vacuoles in
Arabidopsis
actin cytoskeleton
cell biology
cell size
compactness
plant growth
vacuolar occupancy
vacuole
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
25 Sep 2019
25 Sep 2019
Historique:
received:
22
08
2019
revised:
20
09
2019
accepted:
23
09
2019
entrez:
28
9
2019
pubmed:
29
9
2019
medline:
12
2
2020
Statut:
epublish
Résumé
The dimension of the plants largest organelle-the vacuole-plays a major role in defining cellular elongation rates. The morphology of the vacuole is controlled by the actin cytoskeleton, but molecular players remain largely unknown. Recently, the Networked (NET) family of membrane-associated, actin-binding proteins has been identified. Here, we show that NET4A localizes to highly constricted regions of the vacuolar membrane and contributes to vacuolar morphology. Using genetic interference, we found that deregulation of NET4 abundance increases vacuolar occupancy, and that overexpression of NET4 abundance decreases vacuolar occupancy. Our data reveal that NET4A induces more compact vacuoles, correlating with reduced cellular and organ growth in
Identifiants
pubmed: 31557830
pii: ijms20194752
doi: 10.3390/ijms20194752
pmc: PMC6801981
pii:
doi:
Substances chimiques
Arabidopsis Proteins
0
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : SCHE 1836/4-1
Organisme : Vienna Science and Technology Fund
ID : J.K-V.
Organisme : European Research Council
ID : Starting grant 639478
Pays : International
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