Regulation of melanocortin-1 receptor pharmacology by melanocortin receptor accessory protein 2 in orange-spotted grouper (Epinephelus coioides).
Adaptor Proteins, Signal Transducing
/ metabolism
Amino Acid Sequence
Animals
Base Sequence
Bass
/ genetics
Cyclic AMP
/ metabolism
Fish Proteins
/ metabolism
Gene Expression Regulation
HEK293 Cells
Humans
Ligands
MAP Kinase Signaling System
Phylogeny
Receptor, Melanocortin, Type 1
/ chemistry
Signal Transduction
/ drug effects
Epinephelus coioides
Melanocortin receptor accessory protein 2
Melanocortin-1 receptor
Orange-spotted grouper
Pharmacology
Tissue distribution
Journal
General and comparative endocrinology
ISSN: 1095-6840
Titre abrégé: Gen Comp Endocrinol
Pays: United States
ID NLM: 0370735
Informations de publication
Date de publication:
01 01 2020
01 01 2020
Historique:
received:
17
08
2019
revised:
25
09
2019
accepted:
26
09
2019
pubmed:
1
10
2019
medline:
14
3
2020
entrez:
1
10
2019
Statut:
ppublish
Résumé
Melanocortin-1 receptor (MC1R) has important roles in regulating pigmentation and inflammation. Melanocortin receptor accessory protein 2 (MRAP2) modulates trafficking, ligand binding, and signaling of mammalian melanocortin receptors. However, the effect of MRAP2 on fish MC1R has not been extensively studied. Herein, we cloned the orange-spotted grouper (Epinephelus coioides) mc1r, which had a 972 bp open reading frame encoding a putative protein of 323 amino acids. Grouper mc1r was mainly expressed in the brain, skin, testis, spleen, head kidney, and kidney. EcoMC1R showed high constitutive activities in both Gs-cAMP and ERK1/2 pathways, which could be differentially modulated by grouper MRAP2 (EcoMRAP2). Three agonists, including α-melanocyte-stimulating hormone (MSH), β-MSH, and ACTH, could bind to EcoMC1R and dose-dependently increase intracellular cAMP production. EcoMRAP2 had no effect on the IC
Identifiants
pubmed: 31568758
pii: S0016-6480(19)30433-2
doi: 10.1016/j.ygcen.2019.113291
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Fish Proteins
0
Ligands
0
Receptor, Melanocortin, Type 1
0
Cyclic AMP
E0399OZS9N
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113291Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.