Targeted isolation and cultivation of uncultivated bacteria by reverse genomics.
Journal
Nature biotechnology
ISSN: 1546-1696
Titre abrégé: Nat Biotechnol
Pays: United States
ID NLM: 9604648
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
20
01
2019
accepted:
15
08
2019
pubmed:
2
10
2019
medline:
25
1
2020
entrez:
2
10
2019
Statut:
ppublish
Résumé
Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidated. Here, we report an approach to capture specific microorganisms from complex communities into pure cultures using genome-informed antibody engineering. We apply our reverse genomics approach to isolate and sequence single cells and to cultivate three different species-level lineages of human oral Saccharibacteria (TM7). Using our pure cultures, we show that all three Saccharibacteria species are epibionts of diverse Actinobacteria. We also isolate and cultivate human oral SR1 bacteria, which are members of a lineage of previously uncultured bacteria. Reverse-genomics-enabled cultivation of microorganisms can be applied to any species from any environment and has the potential to unlock the isolation, cultivation and characterization of species from as-yet-uncultured branches of the microbial tree of life.
Identifiants
pubmed: 31570900
doi: 10.1038/s41587-019-0260-6
pii: 10.1038/s41587-019-0260-6
pmc: PMC6858544
mid: NIHMS1537601
doi:
Substances chimiques
Antibodies
0
Bacterial Proteins
0
Membrane Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1314-1321Subventions
Organisme : NIDCR NIH HHS
ID : R01 DE024463
Pays : United States
Organisme : NIDCR NIH HHS
ID : R56 DE021567
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : CommentIn
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