Site-Specific Sequential Protein Labeling Catalyzed by a Single Recombinant Ligase.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
30 10 2019
30 10 2019
Historique:
pubmed:
2
10
2019
medline:
5
11
2020
entrez:
2
10
2019
Statut:
ppublish
Résumé
Protein ligases of defined substrate specificity are versatile tools for protein engineering. Upon completion of the reaction, the products of currently reported protein ligases contain the amino acid sequence that is recognized by that same ligase, resulting in repeated cycles of ligation and hydrolysis as competing reactions. Thus, previous efforts to sequentially label proteins at distinct positions required ligases of orthogonal specificity. A recombinant
Identifiants
pubmed: 31573802
doi: 10.1021/jacs.9b09166
pmc: PMC7372569
mid: NIHMS1606810
doi:
Substances chimiques
Proteins
0
Recombinant Proteins
0
Single-Domain Antibodies
0
Green Fluorescent Proteins
147336-22-9
Cysteine Endopeptidases
EC 3.4.22.-
asparaginylendopeptidase
EC 3.4.22.34
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17388-17393Subventions
Organisme : NIAID NIH HHS
ID : R01 AI087879
Pays : United States
Références
Proc Natl Acad Sci U S A. 2019 Jun 11;116(24):11737-11746
pubmed: 31123145
Nat Commun. 2018 Jun 20;9(1):2411
pubmed: 29925835
J Am Chem Soc. 2017 Apr 19;139(15):5351-5358
pubmed: 28199119
Angew Chem Int Ed Engl. 2015 Dec 21;54(52):15694-8
pubmed: 26563575
J Biol Chem. 2009 Jun 5;284(23):16028-36
pubmed: 19359246
Nat Chem Biol. 2014 Sep;10(9):732-8
pubmed: 25038786
Chem Biol. 2015 May 21;22(5):571-82
pubmed: 25960260
Chem Soc Rev. 2018 Dec 21;47(24):9106-9136
pubmed: 30259933
J Biol Chem. 2018 Jun 8;293(23):8934-8946
pubmed: 29628443
J Am Chem Soc. 2008 Dec 3;130(48):16338-43
pubmed: 18989959
Bioconjug Chem. 2018 Oct 17;29(10):3245-3249
pubmed: 30231608
Mol Immunol. 2019 Oct;114:513-523
pubmed: 31518855
Plant J. 2019 Jun;98(6):988-999
pubmed: 30790358
J Am Chem Soc. 2009 Aug 12;131(31):10800-1
pubmed: 19610623
Proc Natl Acad Sci U S A. 2019 Apr 16;116(16):7831-7836
pubmed: 30944220
New Phytol. 2018 May;218(3):923-928
pubmed: 28322452
Chem Rev. 2015 Mar 11;115(5):2174-95
pubmed: 25700113
Angew Chem Int Ed Engl. 2012 Sep 10;51(37):9377-80
pubmed: 22890696
Nat Commun. 2015 Dec 18;6:10199
pubmed: 26680698