Tracking of GFP-labeled unrestricted somatic stem cells transplanted in the sepsis mouse model.
Cell tracking
Cell transplantation
Optical imaging
Sepsis
Umbilical cord blood
Journal
Tissue & cell
ISSN: 1532-3072
Titre abrégé: Tissue Cell
Pays: Scotland
ID NLM: 0214745
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
22
05
2019
revised:
15
07
2019
accepted:
30
07
2019
entrez:
5
10
2019
pubmed:
5
10
2019
medline:
12
2
2020
Statut:
ppublish
Résumé
Cell-based therapy provides a promising approach for the treatment of sepsis and related disorders. Fate determination of transplanted cells is the most essential issue for cell therapist. Optical imaging is the reliable, time and cost-effective system for cell tracking. The present study was aimed to apply an optical imaging system for monitoring of GFP-labeled unrestricted somatic stem cells in the sepsis animal model. In vivo imaging showed the most accumulation of intravenously injected cells into the lungs and liver of septic mice. Thereafter, the imaging data were more approved by flow cytometry and immunohistochemistry staining. Cellular localization in septic lungs and liver that observed by optical imaging technique may offer beneficial evidence for designing of sepsis clinical trials.
Identifiants
pubmed: 31582016
pii: S0040-8166(19)30217-4
doi: 10.1016/j.tice.2019.07.009
pii:
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
33-37Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.