The Alternaria Mycotoxin Alternariol Triggers the Immune Response of IL-1β-stimulated, Differentiated Caco-2 Cells.
alternariol
cytokines
inflammation
microRNAs
mycotoxins
Journal
Molecular nutrition & food research
ISSN: 1613-4133
Titre abrégé: Mol Nutr Food Res
Pays: Germany
ID NLM: 101231818
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
03
04
2019
revised:
20
06
2019
pubmed:
5
10
2019
medline:
23
6
2020
entrez:
5
10
2019
Statut:
ppublish
Résumé
Alternariol (AOH), a toxic secondary metabolite of Alternaria spp., may contaminate a broad spectrum of food and feed. Besides its cytotoxic, genotoxic, and estrogenic properties, several studies report the potential of AOH to suppress the rich network of immune responses. The specific effect of AOH on inflammation-related signaling in non-immune cells of the intestinal epithelial layer has, however, not been investigated yet. Since intestinal epithelial cells (IECs) are, compared to underlying cells, exposed to higher concentrations of the ingested mycotoxin, the question is addressed whether immunomodulation by AOH at the gastrointestinal barrier must be considered. The impact of AOH (0.02-40 µm) on inflammatory signaling in either IL-1β-stimulated or non-stimulated differentiated Caco-2 cells is determined. AOH significantly reduces IL-1β transcription after 5 h but shows an increasing tendency on IL-8 transcript levels after long-term exposure (20 h). In IL-1β-stimulated cells, AOH (20-40 µm) augments TNF-α transcripts while repressing IL-8, IL-6, and IL-1β transcription as well as IL-8 secretion. Furthermore, inflammation-related microRNAs miR-16, miR-146a, miR-125b, and miR-155 are altered in response to AOH. The obtained data indicate that AOH represses immune responses in an inflamed environment, possibly leading to higher susceptibility to diseases.
Identifiants
pubmed: 31584250
doi: 10.1002/mnfr.201900341
pmc: PMC6856692
doi:
Substances chimiques
Cytokines
0
Immunosuppressive Agents
0
Interleukin-1beta
0
Lactones
0
MicroRNAs
0
Mycotoxins
0
alternariol
KN9L4260JW
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1900341Informations de copyright
© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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