Multiparametric radiobiological assays show that variation of X-ray energy strongly impacts relative biological effectiveness: comparison between 220 kV and 4 MV.
Cell Survival
/ radiation effects
Colony-Forming Units Assay
DNA Breaks, Double-Stranded
/ radiation effects
Genetic Markers
/ radiation effects
Histones
/ genetics
Human Umbilical Vein Endothelial Cells
Humans
Linear Energy Transfer
Photons
/ adverse effects
Proof of Concept Study
Relative Biological Effectiveness
X-Rays
/ adverse effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 10 2019
04 10 2019
Historique:
received:
19
06
2019
accepted:
20
09
2019
entrez:
6
10
2019
pubmed:
6
10
2019
medline:
12
11
2020
Statut:
epublish
Résumé
Based on classic clonogenic assay, it is accepted by the scientific community that, whatever the energy, the relative biological effectiveness of X-rays is equal to 1. However, although X-ray beams are widely used in diagnosis, interventional medicine and radiotherapy, comparisons of their energies are scarce. We therefore assessed in vitro the effects of low- and high-energy X-rays using Human umbilical vein endothelial cells (HUVECs) by performing clonogenic assay, measuring viability/mortality, counting γ-H2AX foci, studying cell proliferation and cellular senescence by flow cytometry and by performing gene analysis on custom arrays. Taken together, excepted for γ-H2AX foci counts, these experiments systematically show more adverse effects of high energy X-rays, while the relative biological effectiveness of photons is around 1, whatever the quality of the X-ray beam. These results strongly suggest that multiparametric analysis should be considered in support of clonogenic assay.
Identifiants
pubmed: 31586152
doi: 10.1038/s41598-019-50908-4
pii: 10.1038/s41598-019-50908-4
pmc: PMC6778087
doi:
Substances chimiques
Genetic Markers
0
H2AX protein, human
0
Histones
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
14328Références
Radiat Res. 2016 Mar;185(3):246-56
pubmed: 26930380
Br J Radiol. 2018 Sep;91(1089):20170762
pubmed: 29630386
Aging (Albany NY). 2011 Feb;3(2):168-74
pubmed: 21325706
IEEE Trans Pattern Anal Mach Intell. 1986 Jun;8(6):679-98
pubmed: 21869365
J Exp Med. 1956 May 1;103(5):653-66
pubmed: 13319584
Mutat Res. 2004 Nov 22;556(1-2):209-15
pubmed: 15491649
Radiat Res. 2015 Jan;183(1):82-93
pubmed: 25536232
Mol Cell. 2018 Aug 16;71(4):581-591.e5
pubmed: 30057196
Med Phys. 2009 Aug;36(8):3381-4
pubmed: 19746770
Radiat Oncol. 2014 Jan 14;9:26
pubmed: 24422711
Nat Protoc. 2009;4(12):1798-806
pubmed: 20010931
Radiat Res. 2005 May;163(5):510-9
pubmed: 15850412
PLoS One. 2012;7(4):e35740
pubmed: 22563394
Trends Cell Biol. 2018 Jun;28(6):436-453
pubmed: 29477613
Cell Cycle. 2010 Feb 15;9(4):662-9
pubmed: 20139725
Nat Rev Mol Cell Biol. 2007 Sep;8(9):729-40
pubmed: 17667954
Free Radic Biol Med. 2017 Jul;108:750-759
pubmed: 28431961
Nat Protoc. 2006;1(5):2315-9
pubmed: 17406473
Ann ICRP. 2003;33(4):1-117
pubmed: 14614921
Cytometry. 1994 Oct 1;17(2):185-9
pubmed: 7530620
Acta Oncol. 2017 Nov;56(11):1374-1378
pubmed: 28820311
Methods Mol Med. 2005;110:21-8
pubmed: 15901923
Cell Mol Life Sci. 2014 Nov;71(22):4373-86
pubmed: 25080110
Free Radic Res. 2010 Jun;44(6):587-604
pubmed: 20370560
Mutagenesis. 2012 Nov;27(6):645-52
pubmed: 22914676
Int J Radiat Oncol Biol Phys. 1998 Aug 1;42(1):191-203
pubmed: 9747838
Methods Mol Biol. 2007;371:21-31
pubmed: 17634571
Radiat Environ Biophys. 2003 Apr;42(1):55-61
pubmed: 12720002
J Radiat Res. 2018 May 1;59(3):253-260
pubmed: 29800455
Int J Radiat Oncol Biol Phys. 2017 Oct 1;99(2):353-361
pubmed: 28479002
Radiat Oncol. 2014 Dec 18;9:266
pubmed: 25518850
Mutat Res. 2017 Mar;797-799:15-25
pubmed: 28340407
Int J Radiat Oncol Biol Phys. 2000 Jan 1;46(1):13-9
pubmed: 10656366
Radiat Prot Dosimetry. 2004;112(4):471-81
pubmed: 15623881
J Vis Exp. 2011 Mar 13;(49):
pubmed: 21445039
Strahlenther Onkol. 1999 Jun;175 Suppl 2:39-43
pubmed: 10394395
Int J Radiat Biol. 2018 Jun;94(6):597-606
pubmed: 29701998
PLoS One. 2016 Jan 04;11(1):e0145786
pubmed: 26727594