Enhanced GII.4 human norovirus infection in gnotobiotic pigs transplanted with a human gut microbiota.
Animals
Blood
/ virology
Caliciviridae Infections
/ complications
Cluster Analysis
DNA, Bacterial
/ chemistry
DNA, Ribosomal
/ chemistry
Disease Models, Animal
Duodenum
/ virology
Dysbiosis
Fecal Microbiota Transplantation
Gastrointestinal Microbiome
Genotype
Germ-Free Life
Humans
Ileum
/ virology
Microbial Interactions
Microbiota
Norovirus
/ classification
Phylogeny
RNA, Ribosomal, 16S
/ genetics
Sequence Analysis, DNA
Swine
Time Factors
Viral Load
Virus Shedding
Human norovirus
diarrhea
faecal microbiota transplantation
gnotobiotic pig
human gut microbiota
Journal
The Journal of general virology
ISSN: 1465-2099
Titre abrégé: J Gen Virol
Pays: England
ID NLM: 0077340
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
pubmed:
10
10
2019
medline:
10
6
2020
entrez:
10
10
2019
Statut:
ppublish
Résumé
The role of commensal microbiota in enteric viral infections has been explored extensively, but the interaction between human gut microbiota (HGM) and human norovirus (HuNoV) is poorly understood. In this study, we established an HGM-Transplanted gnotobiotic (Gn) pig model of HuNoV infection and disease, using an infant stool as HGM transplant and a HuNoV GII.4/2006b strain for virus inoculation. Compared to germ-free Gn pigs, HuNoV inoculation in HGMT Gn pigs resulted in increased HuNoV shedding, characterized by significantly higher shedding titres on post inoculation day (PID) 3, 4, 6, 8 and 9, and significantly longer mean duration of virus shedding. In addition, virus titres were significantly higher in duodenum and distal ileum of HGMT Gn pigs on PID10, while comparable and transient HuNoV viremia was detected in both groups. 16S rRNA gene sequencing demonstrated that HuNoV infection dramatically altered intestinal microbiota in HGMT Gn pigs at the phylum (Proteobacteria, Firmicutes and Bacteroidetes) and genus (
Identifiants
pubmed: 31596195
doi: 10.1099/jgv.0.001336
pmc: PMC7137776
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Ribosomal
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1530-1540Subventions
Organisme : NIAID NIH HHS
ID : R01 AI089634
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI123661
Pays : United States
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