Developmental validation of the Microreader™ 20A ID system.
Capillary electrophoresis
Forensic science
Microreader™ 20A ID system
Short tandem repeat (STR)
Validation
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
20
06
2019
revised:
31
08
2019
accepted:
26
09
2019
pubmed:
10
10
2019
medline:
21
4
2020
entrez:
10
10
2019
Statut:
ppublish
Résumé
The Microreader™ 20A ID system is designed for forensic applications such as personal identification, parentage testing, and research. It includes 13 combined DNA index system (CODIS) short tandem repeat (STR) loci (CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11), three expanded CODIS STR loci (D12S391, D19S433, and D2S1338), three non-CODIS STR loci (D6S1043, Penta D, and Penta E), and the amelogenin locus in one reaction with a six-dye fluorescent (FAM, HEX, TAMAR, ROX, PUR, and QD550) analysis system. In this study, the Microreader™ 20A ID system was validated according to the Scientific Working Group on DNA Analysis Methods validation guidelines for forensic DNA Analysis methods and Chinese national standard, including PCR-based studies, sensitivity study, precision, and accuracy evaluation, stutter calculation, inhibitor tests, species specificity, and DNA mixture studies. Our results suggest that the Microreader™ 20A ID system is a useful tool for personal identification and parentage testing.
Identifiants
pubmed: 31596501
doi: 10.1002/elps.201900221
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3099-3107Subventions
Organisme : Applied Basic Research Programs of Science and Technology Department Foundation of Sichuan Province of China
ID : 2017JY0233
Pays : International
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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