Differential Modulation of Innate Immune Responses in Human Primary Cells by Influenza A Viruses Carrying Human or Avian Nonstructural Protein 1.
Animals
Birds
Dendritic Cells
/ immunology
Dogs
Epithelial Cells
/ immunology
Gene Expression Regulation
Host Specificity
Host-Pathogen Interactions
/ genetics
Humans
Immunity, Innate
Influenza A Virus, H1N1 Subtype
/ classification
Influenza A Virus, H3N2 Subtype
/ classification
Influenza A Virus, H5N1 Subtype
/ classification
Influenza A Virus, H7N2 Subtype
/ classification
Influenza A Virus, H7N9 Subtype
/ classification
Interferon-alpha
/ genetics
Interferon-beta
/ genetics
Interferon-gamma
/ genetics
Interleukin-6
/ genetics
Madin Darby Canine Kidney Cells
Phylogeny
Primary Cell Culture
Reassortant Viruses
/ genetics
Tumor Necrosis Factor-alpha
/ genetics
Viral Nonstructural Proteins
/ classification
NS1 protein
dendritic cells
epithelial cells
influenza
innate immunity
interferons
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
12 12 2019
12 12 2019
Historique:
received:
10
07
2019
accepted:
29
09
2019
pubmed:
11
10
2019
medline:
12
6
2020
entrez:
11
10
2019
Statut:
epublish
Résumé
The influenza A virus (IAV) nonstructural protein 1 (NS1) contributes to disease pathogenesis through the inhibition of host innate immune responses. Dendritic cells (DCs) release interferons (IFNs) and proinflammatory cytokines and promote adaptive immunity upon viral infection. In order to characterize the strain-specific effects of IAV NS1 on human DC activation, we infected human DCs with a panel of recombinant viruses with the same backbone (A/Puerto Rico/08/1934) expressing different NS1 proteins from human and avian origin. We found that these viruses induced a clearly distinct phenotype in DCs. Specifically, viruses expressing NS1 from human IAV (either H1N1 or H3N2) induced higher levels of expression of type I (IFN-α and IFN-β) and type III (IFN-λ1 to IFNλ3) IFNs than viruses expressing avian IAV NS1 proteins (H5N1, H7N9, and H7N2), but the differences observed in the expression levels of proinflammatory cytokines like tumor necrosis factor alpha (TNF-α) or interleukin-6 (IL-6) were not significant. In addition, using imaging flow cytometry, we found that human and avian NS1 proteins segregate based on their subcellular trafficking dynamics, which might be associated with the different innate immune profile induced in DCs by viruses expressing those NS1 proteins. Innate immune responses induced by our panel of IAV recombinant viruses were also characterized in normal human bronchial epithelial cells, and the results were consistent with those in DCs. Altogether, our results reveal an increased ability of NS1 from avian viruses to antagonize innate immune responses in human primary cells compared to the ability of NS1 from human viruses, which could contribute to the severe disease induced by avian IAV in humans.
Identifiants
pubmed: 31597767
pii: JVI.00999-19
doi: 10.1128/JVI.00999-19
pmc: PMC6912104
pii:
doi:
Substances chimiques
IL6 protein, human
0
INS1 protein, influenza virus
0
Interferon-alpha
0
Interleukin-6
0
Tumor Necrosis Factor-alpha
0
Viral Nonstructural Proteins
0
Interferon-beta
77238-31-4
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : HHSN272201400008C
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI117873
Pays : United States
Informations de copyright
Copyright © 2019 Monteagudo et al.
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