ProCarbDB: a database of carbohydrate-binding proteins.


Journal

Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011

Informations de publication

Date de publication:
08 01 2020
Historique:
accepted: 05 10 2019
revised: 12 09 2019
received: 01 08 2019
pubmed: 11 10 2019
medline: 30 5 2020
entrez: 11 10 2019
Statut: ppublish

Résumé

Carbohydrate-binding proteins play crucial roles across all organisms and viruses. The complexity of carbohydrate structures, together with inconsistencies in how their 3D structures are reported, has led to difficulties in characterizing the protein-carbohydrate interfaces. In order to better understand protein-carbohydrate interactions, we have developed an open-access database, ProCarbDB, which, unlike the Protein Data Bank (PDB), clearly distinguishes between the complete carbohydrate ligands and their monomeric units. ProCarbDB is a comprehensive database containing over 5200 3D X-ray crystal structures of protein-carbohydrate complexes. In ProCarbDB, the complete carbohydrate ligands are annotated and all their interactions are displayed. Users can also select any protein residue in the proximity of the ligand to inspect its interactions with the carbohydrate ligand and with other neighbouring protein residues. Where available, additional curated information on the binding affinity of the complex and the effects of mutations on the binding have also been provided in the database. We believe that ProCarbDB will be an invaluable resource for understanding protein-carbohydrate interfaces. The ProCarbDB web server is freely available at http://www.procarbdb.science/procarb.

Identifiants

pubmed: 31598690
pii: 5584527
doi: 10.1093/nar/gkz860
pmc: PMC6943041
doi:

Substances chimiques

Ligands 0
Proteins 0
Receptors, Cell Surface 0
saccharide-binding proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

D368-D375

Subventions

Organisme : Wellcome Trust
ID : 200814/Z/16/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M026302/1
Pays : United Kingdom

Informations de copyright

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

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Auteurs

Liviu Copoiu (L)

Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1GA, UK.

Pedro H M Torres (PHM)

Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1GA, UK.

David B Ascher (DB)

Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1GA, UK.
Department of Biochemistry, University of Melbourne, Flemington Road, Parkville, Australia.

Tom L Blundell (TL)

Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1GA, UK.

Sony Malhotra (S)

Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1GA, UK.

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Classifications MeSH