PRX2 and PRX25, peroxidases regulated by COG1, are involved in seed longevity in Arabidopsis.
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Cell Wall
/ metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Germination
/ genetics
Lignin
Lipid Metabolism
Lipids
Membrane Lipids
Mutation
Peroxidases
/ genetics
Proanthocyanidins
Seeds
/ genetics
Transcription Factors
/ metabolism
RNA-seq
Sudan
TEM
accelerating aging
tetrazolium salts
Journal
Plant, cell & environment
ISSN: 1365-3040
Titre abrégé: Plant Cell Environ
Pays: United States
ID NLM: 9309004
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
01
08
2019
accepted:
17
09
2019
pubmed:
11
10
2019
medline:
22
12
2020
entrez:
11
10
2019
Statut:
ppublish
Résumé
Permeability is a crucial trait that affects seed longevity and is regulated by different polymers including proanthocyanidins, suberin, cutin and lignin located in the seed coat. By testing mutants in suberin transport and biosynthesis, we demonstrate the importance of this biopolymer to cope with seed deterioration. Transcriptomic analysis of cog1-2D, a gain-of-function mutant with increased seed longevity, revealed the upregulation of several peroxidase genes. Reverse genetics analysing seed longevity uncovered redundancy within the seed coat peroxidase gene family; however, after controlled deterioration treatment, seeds from the prx2 prx25 double and prx2 prx25 prx71 triple mutant plants presented lower germination than wild-type plants. Transmission electron microscopy analysis of the seed coat of these mutants showed a thinner palisade layer, but no changes were observed in proanthocyanidin accumulation or in the cuticle layer. Spectrophotometric quantification of acetyl bromide-soluble lignin components indicated changes in the amount of total polyphenolics derived from suberin and/or lignin in the mutant seeds. Finally, the increased seed coat permeability to tetrazolium salts observed in the prx2 prx25 and prx2 prx25 prx71 mutant lines suggested that the lower permeability of the seed coats caused by altered polyphenolics is likely to be the main reason explaining their reduced seed longevity.
Substances chimiques
Arabidopsis Proteins
0
COG1 protein, Arabidopsis
0
Lipids
0
Membrane Lipids
0
Proanthocyanidins
0
Transcription Factors
0
proanthocyanidin
18206-61-6
cutin
54990-88-4
suberin
8072-95-5
Lignin
9005-53-2
Peroxidases
EC 1.11.1.-
Prx2 protein, Arabidopsis
EC 1.11.1.-
Prx25 protein, Arabidopsis
EC 1.11.1.-
Prx71 protein, Arabidopsis
EC 1.11.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
315-326Informations de copyright
© 2019 John Wiley & Sons Ltd.
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