Vector competence is strongly affected by a small deletion or point mutations in bluetongue virus.
Arbovirus
Bluetongue virus
Culicoides
Feeding model
Midge
Vector competence
Virus propagation
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
11 Oct 2019
11 Oct 2019
Historique:
received:
20
06
2019
accepted:
16
09
2019
entrez:
13
10
2019
pubmed:
13
10
2019
medline:
15
1
2020
Statut:
epublish
Résumé
Transmission of vector-borne virus by insects is a complex mechanism consisting of many different processes; viremia in the host, uptake, infection and dissemination in the vector, and delivery of virus during blood-feeding leading to infection of the susceptible host. Bluetongue virus (BTV) is the prototype vector-borne orbivirus (family Reoviridae). BTV serotypes 1-24 (typical BTVs) are transmitted by competent biting Culicoides midges and replicate in mammalian (BSR) and midge (KC) cells. Previously, we showed that genome segment 10 (S10) encoding NS3/NS3a protein is required for virus propagation in midges. BTV serotypes 25-27 (atypical BTVs) do not replicate in KC cells. Several distinct BTV26 genome segments cause this so-called 'differential virus replication' in vitro. Virus strains were generated using reverse genetics and their growth was examined in vitro. The midge feeding model has been developed to study infection, replication and disseminations of virus in vivo. A laboratory colony of C. sonorensis, a known competent BTV vector, was fed or injected with BTV variants and propagation in the midge was examined using PCR testing. Crossing of the midgut infection barrier was examined by separate testing of midge heads and bodies. A 100 nl blood meal containing ±10 Small changes in NS3/NS3a or in the outer shell protein VP2 strongly affect virus propagation in midges and thus vector competence. Therefore, spread of disease by competent Culicoides midges can strongly differ for very closely related viruses.
Sections du résumé
BACKGROUND
BACKGROUND
Transmission of vector-borne virus by insects is a complex mechanism consisting of many different processes; viremia in the host, uptake, infection and dissemination in the vector, and delivery of virus during blood-feeding leading to infection of the susceptible host. Bluetongue virus (BTV) is the prototype vector-borne orbivirus (family Reoviridae). BTV serotypes 1-24 (typical BTVs) are transmitted by competent biting Culicoides midges and replicate in mammalian (BSR) and midge (KC) cells. Previously, we showed that genome segment 10 (S10) encoding NS3/NS3a protein is required for virus propagation in midges. BTV serotypes 25-27 (atypical BTVs) do not replicate in KC cells. Several distinct BTV26 genome segments cause this so-called 'differential virus replication' in vitro.
METHODS
METHODS
Virus strains were generated using reverse genetics and their growth was examined in vitro. The midge feeding model has been developed to study infection, replication and disseminations of virus in vivo. A laboratory colony of C. sonorensis, a known competent BTV vector, was fed or injected with BTV variants and propagation in the midge was examined using PCR testing. Crossing of the midgut infection barrier was examined by separate testing of midge heads and bodies.
RESULTS
RESULTS
A 100 nl blood meal containing ±10
CONCLUSION
CONCLUSIONS
Small changes in NS3/NS3a or in the outer shell protein VP2 strongly affect virus propagation in midges and thus vector competence. Therefore, spread of disease by competent Culicoides midges can strongly differ for very closely related viruses.
Identifiants
pubmed: 31604476
doi: 10.1186/s13071-019-3722-2
pii: 10.1186/s13071-019-3722-2
pmc: PMC6790033
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
470Subventions
Organisme : Dutch ministry of Agriculture, Nature and Food Quality
ID : WOT-01-003-079
Organisme : Dutch ministry of Agriculture, Nature and Food Quality
ID : KB-21-006-030
Organisme : Agricultural Research Service
ID : 3020-32000-010
Références
J Med Entomol. 1974 Jul 15;11(3):316-23
pubmed: 4368984
Biopolymers. 2007 May;86(1):83-94
pubmed: 17323325
J Gen Virol. 2009 Jun;90(Pt 6):1423-32
pubmed: 19264638
PLoS One. 2014 Mar 21;9(3):e92377
pubmed: 24658296
Vaccine. 2018 Apr 5;36(15):1925-1933
pubmed: 29525278
PLoS One. 2016 Feb 18;11(2):e0149709
pubmed: 26890863
Vaccine. 2018 Jun 14;36(25):3584-3592
pubmed: 29759377
Transbound Emerg Dis. 2018 Apr;65(2):e251-e263
pubmed: 29243405
Arch Virol. 1977;54(4):333-43
pubmed: 562143
Emerg Infect Dis. 2014 Dec;20(12):2123-5
pubmed: 25418049
Transbound Emerg Dis. 2018 Apr;65(2):370-374
pubmed: 29392882
Vet Rec. 2008 Aug 16;163(7):203-9
pubmed: 18708653
J Virol. 2012 Aug;86(15):7858-66
pubmed: 22593166
J Gen Virol. 2007 Feb;88(Pt 2):621-30
pubmed: 17251581
Arch Med Res. 2002 Jul-Aug;33(4):330-42
pubmed: 12234522
J Virol Methods. 2007 Mar;140(1-2):115-23
pubmed: 17196266
Vet Microbiol. 2009 Sep 18;138(3-4):235-43
pubmed: 19419822
Transbound Emerg Dis. 2014 Feb;61(1):69-74
pubmed: 22937914
J Invertebr Pathol. 1989 Nov;54(3):385-93
pubmed: 2553822
Vet Microbiol. 2009 Jul 2;138(1-2):11-9
pubmed: 19272719
Vet Microbiol. 2016 Aug 30;192:145-151
pubmed: 27527776
Antiviral Res. 2010 Feb;85(2):328-45
pubmed: 19857523
Virus Res. 2010 Aug;151(2):109-17
pubmed: 20621672
PLoS One. 2012;7(2):e30540
pubmed: 22363444
PLoS One. 2014 May 05;9(5):e96049
pubmed: 24797910
Parasit Vectors. 2015 Sep 17;8:476
pubmed: 26383094
J Virol. 2006 Jan;80(1):460-73
pubmed: 16352570
Transbound Emerg Dis. 2019 May;66(3):1177-1185
pubmed: 30661301
Virol J. 2016 Jul 02;13:119
pubmed: 27368544
J Gen Virol. 2014 Sep;95(Pt 9):2019-29
pubmed: 24914064
J Vet Diagn Invest. 2012 May;24(3):469-78
pubmed: 22529113
Crit Rev Microbiol. 2017 Mar;43(2):142-155
pubmed: 27800699
Res Vet Sci. 1979 Jul;27(1):118-20
pubmed: 228365
J Virol. 2017 Jan 31;91(4):
pubmed: 27903804
J Virol Methods. 2010 Feb;163(2):175-85
pubmed: 19770004
J Virol. 2008 Sep;82(17):8339-48
pubmed: 18562540
J Antimicrob Chemother. 2011 Mar;66(3):466-70
pubmed: 21172786
J Gen Virol. 2005 Jun;86(Pt 6):1801-5
pubmed: 15914859
Emerg Infect Dis. 2008 Dec;14(12):1855-61
pubmed: 19046507
PLoS Pathog. 2009 Mar;5(3):e1000345
pubmed: 19300491
J Med Entomol. 1990 Sep;27(5):934-7
pubmed: 2231631
Vet Microbiol. 2010 Feb 24;141(1-2):31-5
pubmed: 19713058
Curr Opin Virol. 2017 Jun;24:115-123
pubmed: 28609677
J Vet Diagn Invest. 2015 Jul;27(4):442-8
pubmed: 26069226
Nat Struct Mol Biol. 2016 Jan;23(1):74-80
pubmed: 26641711
Virology. 2008 Aug 1;377(2):308-18
pubmed: 18570969
PLoS One. 2014 Jan 20;9(1):e85788
pubmed: 24465709
PLoS One. 2011;6(10):e26147
pubmed: 22031822