Identification of immunoreactive linear epitopes of Borrelia miyamotoi.

Borrelia / physiology Epitopes / isolation & purification Serologic Tests / instrumentation
Borrelia miyamotoi Diagnostics Peptide array Serology

Journal

Ticks and tick-borne diseases
ISSN: 1877-9603
Titre abrégé: Ticks Tick Borne Dis
Pays: Netherlands
ID NLM: 101522599

Informations de publication

Date de publication:
01 2020
Historique:
received: 22 08 2019
revised: 30 09 2019
accepted: 13 10 2019
pubmed: 23 10 2019
medline: 9 3 2021
entrez: 23 10 2019
Statut: ppublish

Résumé

Borrelia miyamotoi is an emerging tick-borne spirochete transmitted by ixodid ticks. Current serologic assays for B. miyamotoi are impacted by genetic similarities to other Borrelia and limited understanding of optimal antigenic targets. In this study, we employed the TBD-Serochip, a peptide array platform, to identify new linear targets for serologic detection of B. miyamotoi. We examined a wide range of suspected B. miyamotoi antigens and identified 352 IgM and 91 IgG reactive peptides, with the majority mapping to variable membrane proteins. These included peptides within conserved fragments of variable membrane proteins that may have greater potential for differential diagnosis. We also identified reactive regions on FlaB, and demonstrate crossreactivity of B. burgdorferi s.l. C6 with a B. miyamotoi C6-like peptide. The panel of linear peptides identified in this study can be used to enhance serodiagnosis of B. miyamotoi.

Identifiants

DOI: 10.1016/j.ttbdis.2019.101314 PMID: 31636001
pubmed: 31636001
pii: S1877-959X(19)30353-X
doi: 10.1016/j.ttbdis.2019.101314
pii:
doi:

Substances chimiques

Epitopes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101314

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier GmbH.. All rights reserved.

Auteurs

Rafal Tokarz (R)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, United States; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, United States. Electronic address: rt2249@columbia.edu.

Teresa Tagliafierro (T)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, United States.

Adrian Caciula (A)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, United States.

Nischay Mishra (N)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, United States; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, United States.

Riddhi Thakkar (R)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, United States.

Lokendra V Chauhan (LV)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, United States.

Stephen Sameroff (S)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, United States.

Shannon Delaney (S)

Department of Psychiatry, Columbia University, New York, NY, United States.

Gary P Wormser (GP)

Division of Infectious Diseases, New York Medical College, Valhalla, NY, United States.

Adriana Marques (A)

Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States.

W Ian Lipkin (WI)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, United States; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, United States.

Articles similaires

Generation of binder-format-payload conjugate-matrices by antibody chain-exchange.

Vedran Vasic, Steffen Dickopf, Nadine Spranger et al.
1.00
Immunoconjugates Humans Antibodies, Bispecific Receptor, ErbB-2 Cell Line, Tumor

Structural insights into epitope-paratope interactions of a monoclonal antibody targeting CEACAM5-expressing tumors.

Anand Kumar, Francis Duffieux, Marie Gagnaire et al.
1.00
Humans Epitopes Antibodies, Monoclonal Immunoglobulin Fab Fragments Cryoelectron Microscopy

Serological and molecular diagnosis of Trypanosoma vivax on buffalos (Bubalus bubalis) and their ectoparasites in the lowlands of Maranhão, Brazil.

Thais Bastos Rocha Serra, Andrea Teles Dos Reis, Carla Fernanda do Carmo Silva et al.
1.00
Animals Brazil Buffaloes Trypanosoma vivax Trypanosomiasis, African

Trend in serological and molecular diagnostic methods for Toxoplasma gondii infection.

Min-Ju Kim, Soeun J Park, Hyunwoo Park
1.00
Humans Toxoplasmosis Toxoplasma Serologic Tests Molecular Diagnostic Techniques

Classifications MeSH

questionsmedicales.fr › Bactéries › Spirochaetales › Spirochaetaceae › Borrelia › Identification of immunoreactive linear...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Épitopes › Identification of immunoreactive linear...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Tests sérologiques › Identification of immunoreactive linear...