SERPINB12 as a possible marker of steroid dependency in children with eosinophilic esophagitis: A pilot study.
Adolescent
Biomarkers
Budesonide
/ administration & dosage
Child
Eosinophilic Esophagitis
/ drug therapy
Esophagus
/ pathology
Female
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Humans
Male
Pilot Projects
RNA, Messenger
/ genetics
Recurrence
Serpins
/ genetics
Steroids
/ administration & dosage
Eosinophilic esophagitis
Next-generation sequencing
SERPINB12
Steroid dependency
mRNA expression profile
Journal
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
ISSN: 1878-3562
Titre abrégé: Dig Liver Dis
Pays: Netherlands
ID NLM: 100958385
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
10
04
2019
revised:
22
06
2019
accepted:
15
08
2019
pubmed:
28
10
2019
medline:
16
12
2020
entrez:
27
10
2019
Statut:
ppublish
Résumé
Topical steroids are effective in eosinophilic esophagitis (EoE), but patients often show different tendencies to relapse. We assessed whether gene expression is associated with a sort of steroid dependency in EoE children. Biopsy samples were prospectively collected on EoE children responding to topical steroids. Patients treated with viscous budesonide for 24 weeks were subsequently classified as early (6 months) or late (>6 months) relapsing. RNA was isolated from esophageal biopsies at the time of the relapse and analyzed by NGS for transcriptome profiling. Of 40 patients, 22 patients were considered for mRNA expression profile. Thirteen were included in the early-relapse group, and 9 were in the late-relapse. No significant difference was observed in the two groups for clinical, endoscopic or histological features. Using the mRNA expression profile we performed supervised clustering using the 10 top differentially expressed genes between early and late relapsing patients. The heatmap and PCA show a proper segregation among patients. SERPINB12 is the only gene attaining a significant differential expression between the two groups (FDR < 0.05). Different tendencies to relapse in EoE children responding to topical steroids might be related to altered mRNA expressions. SERPINB12 presented a significantly higher expression in the late relapse group and it deserves further investigations.
Sections du résumé
BACKGROUND
Topical steroids are effective in eosinophilic esophagitis (EoE), but patients often show different tendencies to relapse. We assessed whether gene expression is associated with a sort of steroid dependency in EoE children.
METHODS
Biopsy samples were prospectively collected on EoE children responding to topical steroids. Patients treated with viscous budesonide for 24 weeks were subsequently classified as early (6 months) or late (>6 months) relapsing. RNA was isolated from esophageal biopsies at the time of the relapse and analyzed by NGS for transcriptome profiling.
RESULTS
Of 40 patients, 22 patients were considered for mRNA expression profile. Thirteen were included in the early-relapse group, and 9 were in the late-relapse. No significant difference was observed in the two groups for clinical, endoscopic or histological features. Using the mRNA expression profile we performed supervised clustering using the 10 top differentially expressed genes between early and late relapsing patients. The heatmap and PCA show a proper segregation among patients. SERPINB12 is the only gene attaining a significant differential expression between the two groups (FDR < 0.05).
CONCLUSIONS
Different tendencies to relapse in EoE children responding to topical steroids might be related to altered mRNA expressions. SERPINB12 presented a significantly higher expression in the late relapse group and it deserves further investigations.
Identifiants
pubmed: 31653522
pii: S1590-8658(19)30781-9
doi: 10.1016/j.dld.2019.08.018
pii:
doi:
Substances chimiques
Biomarkers
0
RNA, Messenger
0
SERPINB12 protein, human
0
Serpins
0
Steroids
0
Budesonide
51333-22-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
158-163Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.