CRISPR/Cas13a-Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification-Free miRNA Diagnostics.
CRISPR-powered biosensing
cancer diagnostics
electrochemical biosensors
microRNAs
microfluidics
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
16
08
2019
revised:
23
09
2019
pubmed:
31
10
2019
medline:
1
5
2020
entrez:
31
10
2019
Statut:
ppublish
Résumé
Noncoding small RNAs, such as microRNAs, are becoming the biomarkers of choice for multiple diseases in clinical diagnostics. A dysregulation of these microRNAs can be associated with many different diseases, such as cancer, dementia, and cardiovascular conditions. The key for effective treatment is an accurate initial diagnosis at an early stage, improving the patient's survival chances. In this work, the first clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a-powered microfluidic, integrated electrochemical biosensor for the on-site detection of microRNAs is introduced. Through this unique combination, the quantification of the potential tumor markers microRNA miR-19b and miR-20a is realized without any nucleic acid amplification. With a readout time of 9 min and an overall process time of less than 4 h, a limit of detection of 10 pm is achieved, using a measuring volume of less than 0.6 µL. Furthermore, the feasibility of the biosensor platform to detect miR-19b in serum samples of children, suffering from brain cancer, is demonstrated. The validation of the obtained results with a standard quantitative real-time polymerase chain reaction method shows the ability of the electrochemical CRISPR-powered system to be a low-cost, easily scalable, and target amplification-free tool for nucleic acid based diagnostics.
Identifiants
pubmed: 31663165
doi: 10.1002/adma.201905311
doi:
Substances chimiques
CRISPR-Associated Proteins
0
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1905311Subventions
Organisme : Austrian Science Fund
ID : I3194-B26
Organisme : German Research Foundation
ID : EXC-294 (BIOSS)
Organisme : German Research Foundation
ID : UR 70/12-01
Organisme : Austrian Science Fund FWF
ID : I 3194
Pays : Austria
Organisme : German Research Foundation
ID : WE 4733/4
Organisme : Medical University of Vienna
ID : 1244/2016
Organisme : German Research Foundation
ID : UR 70/10-01
Informations de copyright
© 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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