Colorimetric Assay Reports on Acyl Carrier Protein Interactions.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 10 2019
30 10 2019
Historique:
received:
16
06
2019
accepted:
25
09
2019
entrez:
1
11
2019
pubmed:
2
11
2019
medline:
11
11
2020
Statut:
epublish
Résumé
The ability to produce new molecules of potential pharmaceutical relevance via combinatorial biosynthesis hinges on improving our understanding of acyl-carrier protein (ACP)-protein interactions. However, the weak and transient nature of these interactions makes them difficult to study using traditional spectroscopic approaches. Herein we report that converting the terminal thiol of the E. coli ACP 4'-phosphopantetheine arm into a mixed disulfide with 2-nitro-5-thiobenzoate ion (TNB
Identifiants
pubmed: 31666546
doi: 10.1038/s41598-019-51554-6
pii: 10.1038/s41598-019-51554-6
pmc: PMC6821831
doi:
Substances chimiques
Acyl Carrier Protein
0
Nitrobenzoates
0
Sulfhydryl Compounds
0
thionitrobenzoic acid
15139-21-6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15589Subventions
Organisme : NIGMS NIH HHS
ID : R15 GM120704
Pays : United States
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