Early Diverging Fungus Mucor circinelloides Lacks Centromeric Histone CENP-A and Displays a Mosaic of Point and Regional Centromeres.
CENP-A
Grem-LINE1
Mis12 complex
Mucoromycotina
RNAi
centromere
early-diverging fungi
kinetochore
mosaic
retrotransposon
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
18 11 2019
18 11 2019
Historique:
received:
26
07
2019
revised:
06
09
2019
accepted:
10
09
2019
pubmed:
5
11
2019
medline:
10
9
2020
entrez:
5
11
2019
Statut:
ppublish
Résumé
Centromeres are rapidly evolving across eukaryotes, despite performing a conserved function to ensure high-fidelity chromosome segregation. CENP-A chromatin is a hallmark of a functional centromere in most organisms. Due to its critical role in kinetochore architecture, the loss of CENP-A is tolerated in only a few organisms, many of which possess holocentric chromosomes. Here, we characterize the consequence of the loss of CENP-A in the fungal kingdom. Mucor circinelloides, an opportunistic human pathogen, lacks CENP-A along with the evolutionarily conserved CENP-C but assembles a monocentric chromosome with a localized kinetochore complex throughout the cell cycle. Mis12 and Dsn1, two conserved kinetochore proteins, were found to co-localize to a short region, one in each of nine large scaffolds, composed of an ∼200-bp AT-rich sequence followed by a centromere-specific conserved motif that echoes the structure of budding yeast point centromeres. Resembling fungal regional centromeres, these core centromere regions are embedded in large genomic expanses devoid of genes yet marked by Grem-LINE1s, a novel retrotransposable element silenced by the Dicer-dependent RNAi pathway. Our results suggest that these hybrid features of point and regional centromeres arose from the absence of CENP-A, thus defining novel mosaic centromeres in this early-diverging fungus.
Identifiants
pubmed: 31679929
pii: S0960-9822(19)31186-8
doi: 10.1016/j.cub.2019.09.024
pmc: PMC6925572
mid: NIHMS1545108
pii:
doi:
Substances chimiques
Centromere Protein A
0
Chromatin
0
Chromosomal Proteins, Non-Histone
0
Histones
0
centromere protein C
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3791-3802.e6Subventions
Organisme : NIAID NIH HHS
ID : R01 AI039115
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI050113
Pays : United States
Organisme : NIAID NIH HHS
ID : R37 AI039115
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
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