The Microtubule Severing Protein Katanin Regulates Proliferation of Neuronal Progenitors in Embryonic and Adult Neurogenesis.
Age Factors
Alleles
Animals
Cell Differentiation
/ genetics
Cell Proliferation
Cerebral Cortex
/ embryology
Dentate Gyrus
/ embryology
Gene Targeting
Haploinsufficiency
Katanin
/ genetics
Learning
Memory
Mice
Mice, Knockout
Microtubules
/ metabolism
Neural Stem Cells
/ cytology
Neurogenesis
/ genetics
Neurons
/ cytology
Organogenesis
Phenotype
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 11 2019
04 11 2019
Historique:
received:
20
06
2019
accepted:
12
10
2019
entrez:
6
11
2019
pubmed:
7
11
2019
medline:
6
11
2020
Statut:
epublish
Résumé
Microtubule severing regulates cytoskeletal rearrangement underlying various cellular functions. Katanin, a heterodimer, consisting of catalytic (p60) and regulatory (p80) subunits severs dynamic microtubules to modulate several stages of cell division. The role of p60 katanin in the mammalian brain with respect to embryonic and adult neurogenesis is poorly understood. Here, we generated a Katna1 knockout mouse and found that consistent with a critical role of katanin in mitosis, constitutive homozygous Katna1 depletion is lethal. Katanin p60 haploinsufficiency induced an accumulation of neuronal progenitors in the subventricular zone during corticogenesis, and impaired their proliferation in the adult hippocampus dentate gyrus (DG) subgranular zone. This did not compromise DG plasticity or spatial and contextual learning and memory tasks employed in our study, consistent with the interpretation that adult neurogenesis may be associated with selective forms of hippocampal-dependent cognitive processes. Our data identify a critical role for the microtubule-severing protein katanin p60 in regulating neuronal progenitor proliferation in vivo during embryonic development and adult neurogenesis.
Identifiants
pubmed: 31685876
doi: 10.1038/s41598-019-52367-3
pii: 10.1038/s41598-019-52367-3
pmc: PMC6828949
doi:
Substances chimiques
Katanin
EC 5.6.1.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15940Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM079421
Pays : United States
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