In vivo generation of haematopoietic stem/progenitor cells from bone marrow-derived haemogenic endothelium.
Animals
Animals, Genetically Modified
Aorta
/ cytology
Bone Marrow Cells
/ cytology
Cell Differentiation
Cell Lineage
/ genetics
Chickens
Embryo, Mammalian
Embryo, Nonmammalian
Female
Fetus
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Regulatory Networks
Hemangioblasts
/ cytology
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
/ cytology
Heterozygote
Homozygote
Male
Mice
Pregnancy
Yolk Sac
/ cytology
Journal
Nature cell biology
ISSN: 1476-4679
Titre abrégé: Nat Cell Biol
Pays: England
ID NLM: 100890575
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
06
11
2018
accepted:
23
09
2019
pubmed:
7
11
2019
medline:
20
2
2020
entrez:
6
11
2019
Statut:
ppublish
Résumé
It is well established that haematopoietic stem and progenitor cells (HSPCs) are generated from a transient subset of specialized endothelial cells termed haemogenic, present in the yolk sac, placenta and aorta, through an endothelial-to-haematopoietic transition (EHT). HSPC generation via EHT is thought to be restricted to the early stages of development. By using experimental embryology and genetic approaches in birds and mice, respectively, we document here the discovery of a bone marrow haemogenic endothelium in the late fetus/young adult. These cells are capable of de novo producing a cohort of HSPCs in situ that harbour a very specific molecular signature close to that of aortic endothelial cells undergoing EHT or their immediate progenies, i.e., recently emerged HSPCs. Taken together, our results reveal that HSPCs can be generated de novo past embryonic stages. Understanding the molecular events controlling this production will be critical for devising innovative therapies.
Identifiants
pubmed: 31685991
doi: 10.1038/s41556-019-0410-6
pii: 10.1038/s41556-019-0410-6
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1334-1345Références
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