Single-cell sequencing and its applications in head and neck cancer.
Diagnostic biomarker
Head and neck cancer
Immunotherapy
Single cell
Single cell RNA sequencing
Single cell sequencing
Squamous cell carcinoma
Technology development
Tumor heterogeneity
Journal
Oral oncology
ISSN: 1879-0593
Titre abrégé: Oral Oncol
Pays: England
ID NLM: 9709118
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
03
09
2019
accepted:
01
10
2019
pubmed:
7
11
2019
medline:
10
9
2020
entrez:
6
11
2019
Statut:
ppublish
Résumé
Head and neck squamous cell carcinoma (HNSCC), like many tumors, is characterized by significant intra-tumoral heterogeneity, namely transcriptional, genetic, and epigenetic differences that define distinct cellular subpopulations. While it has been established that intra-tumoral heterogeneity may have prognostic significance in HNSCC, we are only beginning to describe and define such heterogeneity at a cellular resolution. Recent advances in single-cell sequencing technologies have been critical in this regard, opening new avenues in our understanding of more nuanced tumor biology by identifying distinct cellular subpopulations, dissecting signaling within the tumor microenvironment, and characterizing cellular genomic mutations and copy number aberrations. The combined effect of these insights is likely to be robust and meaningful changes in existing diagnostic and treatment algorithms through the application of novel biomarkers as well as targeted therapeutics. Here, we review single-cell technological and computational advances at the genomic, transcriptomic, and epigenomic levels, and discuss their applications in cancer research and clinical practice, with a specific focus on HNSCC.
Identifiants
pubmed: 31689639
pii: S1368-8375(19)30342-2
doi: 10.1016/j.oraloncology.2019.104441
pmc: PMC6981283
mid: NIHMS1542100
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
104441Subventions
Organisme : NCI NIH HHS
ID : K08 CA237732
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
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