A Glycine max sodium/hydrogen exchanger enhances salt tolerance through maintaining higher Na
GmNHX1
K+/Na+ ratio
Salt stress
Soybean
VIGS
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
05 Nov 2019
05 Nov 2019
Historique:
received:
29
01
2019
accepted:
17
10
2019
entrez:
7
11
2019
pubmed:
7
11
2019
medline:
19
2
2020
Statut:
epublish
Résumé
Soybean (Glycine max (L.)) is one the most important oil-yielding cash crops. However, the soybean production has been seriously restricted by salinization. It is therefore crucial to identify salt tolerance-related genes and reveal molecular mechanisms underlying salt tolerance in soybean crops. A better understanding of how plants resist salt stress provides insights in improving existing soybean varieties as well as cultivating novel salt tolerant varieties. In this study, the biological function of GmNHX1, a NHX-like gene, and the molecular basis underlying GmNHX1-mediated salt stress resistance have been revealed. We found that the transcription level of GmNHX1 was up-regulated under salt stress condition in soybean, reaching its peak at 24 h after salt treatment. By employing the virus-induced gene silencing technique (VIGS), we also found that soybean plants became more susceptible to salt stress after silencing GmNHX1 than wild-type and more silenced plants wilted than wild-type under salt treatment. Furthermore, Arabidopsis thaliana expressing GmNHX1 grew taller and generated more rosette leaves under salt stress condition compared to wild-type. Exogenous expression of GmNHX1 resulted in an increase of Na Vacuolar membrane-localized GmNHX1 enhances plant salt tolerance through maintaining a high K
Sections du résumé
BACKGROUND
BACKGROUND
Soybean (Glycine max (L.)) is one the most important oil-yielding cash crops. However, the soybean production has been seriously restricted by salinization. It is therefore crucial to identify salt tolerance-related genes and reveal molecular mechanisms underlying salt tolerance in soybean crops. A better understanding of how plants resist salt stress provides insights in improving existing soybean varieties as well as cultivating novel salt tolerant varieties. In this study, the biological function of GmNHX1, a NHX-like gene, and the molecular basis underlying GmNHX1-mediated salt stress resistance have been revealed.
RESULTS
RESULTS
We found that the transcription level of GmNHX1 was up-regulated under salt stress condition in soybean, reaching its peak at 24 h after salt treatment. By employing the virus-induced gene silencing technique (VIGS), we also found that soybean plants became more susceptible to salt stress after silencing GmNHX1 than wild-type and more silenced plants wilted than wild-type under salt treatment. Furthermore, Arabidopsis thaliana expressing GmNHX1 grew taller and generated more rosette leaves under salt stress condition compared to wild-type. Exogenous expression of GmNHX1 resulted in an increase of Na
CONCLUSION
CONCLUSIONS
Vacuolar membrane-localized GmNHX1 enhances plant salt tolerance through maintaining a high K
Identifiants
pubmed: 31690290
doi: 10.1186/s12870-019-2084-4
pii: 10.1186/s12870-019-2084-4
pmc: PMC6833268
doi:
Substances chimiques
Plant Proteins
0
Sodium-Hydrogen Exchangers
0
Sodium
9NEZ333N27
Potassium
RWP5GA015D
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
469Subventions
Organisme : Major scientific and technological projects for breeding new varieties of genetically modified organisms
ID : 2009ZX08004-001B
Organisme : Major scientific and technological projects for breeding new varieties of genetically modified organisms
ID : 2014ZX0800402B-001
Organisme : Introducing Foreign Student Funding Program
ID : CN201706
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