Phytoestrogen coumestrol attenuates brain mitochondrial dysfunction and long-term cognitive deficits following neonatal hypoxia-ischemia.
Animals
Brain
/ drug effects
Cognition
/ drug effects
Cognitive Dysfunction
/ drug therapy
Coumestrol
/ pharmacology
Hypoxia-Ischemia, Brain
/ drug therapy
Male
Maze Learning
/ drug effects
Mitochondria
/ drug effects
Neuroprotective Agents
/ pharmacology
Phytoestrogens
/ pharmacology
Rats
Rats, Wistar
Reactive Oxygen Species
/ metabolism
Astrocytes
Coumestrol
Hippocampus
Mitochondria
Neonatal hypoxia-ischemia
Journal
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience
ISSN: 1873-474X
Titre abrégé: Int J Dev Neurosci
Pays: United States
ID NLM: 8401784
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
01
07
2019
revised:
21
10
2019
accepted:
21
10
2019
pubmed:
7
11
2019
medline:
12
5
2020
entrez:
7
11
2019
Statut:
ppublish
Résumé
Neonatal Hypoxia-Ischemia (HI) is a major cause of morbidity and mortality, and is frequently associated with short and long-term neurologic and cognitive impairments. The HI injury causes mitochondrial damage leading to increased production of reactive oxygen species (ROS). Phytoestrogens are non-steroidal plant substances structurally and functionally similar to estrogen. Coumestrol is a potent isoflavonoid with a protective effect against ischemic brain damage in adult rats. Our aim was to determine if coumestrol treatment following neonatal HI attenuates the long-term cognitive deficits induced by neonatal HI, as well as to investigate one possible mechanism underlying its potential effect. On the 7th postnatal day, male Wistar rats were submitted to the Levine-Rice HI model. Intraperitoneal injections of 20 mg/kg of coumestrol, or vehicle, were administered immediately pre-hypoxia or 3 h post-hypoxia. At 12 h after HI the mitochondrial status and ROS levels were determined. At 60th postnatal day the cognitive deficits were revealed in the Morris water maze reference and working spatial memories. Following behavioral analysis, histological assessment was performed and reactive astrogliosis was measured by GFAP expression. Results demonstrate that both pre- and post-HI administration of coumestrol were able to counteract the long-term cognitive and morphological impairments caused by HI, as well as to block the late reactive astrogliosis. The pre-HI administration of coumestrol was able to prevent the early mitochondrial dysfunction in the hippocampus of injured rat pups. Present data suggest that coumestrol exerts protection against experimental neonatal brain hypoxia-ischemia through, at least in part, early modulation of mitochondrial function.
Identifiants
pubmed: 31693927
doi: 10.1016/j.ijdevneu.2019.10.009
doi:
Substances chimiques
Neuroprotective Agents
0
Phytoestrogens
0
Reactive Oxygen Species
0
Coumestrol
V7NW98OB34
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
86-95Informations de copyright
Copyright © 2019 ISDN. Published by Elsevier Ltd. All rights reserved.
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