Application of MALDI-TOF MS to rapid identification of anaerobic bacteria.
Anaerobes
Bacteria identification
MALDI-TOF MS
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
07 Nov 2019
07 Nov 2019
Historique:
received:
17
04
2019
accepted:
21
10
2019
entrez:
9
11
2019
pubmed:
9
11
2019
medline:
15
1
2020
Statut:
epublish
Résumé
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification. To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes. Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0. The identification accuracy of MALDI-TOF MS was 84% for species (I Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria.
Sections du résumé
BACKGROUND
BACKGROUND
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been rapidly developed and widely used as an analytical technique in clinical laboratories with high accuracy in microorganism identification.
OBJECTIVE
OBJECTIVE
To validate the efficacy of MALDI-TOF MS in identification of clinical pathogenic anaerobes.
METHODS
METHODS
Twenty-eight studies covering 6685 strains of anaerobic bacteria were included in this meta-analysis. Fixed-effects models based on the P-value and the I-squared were used for meta-analysis to consider the possibility of heterogeneity between studies. Statistical analyses were performed by using STATA 12.0.
RESULTS
RESULTS
The identification accuracy of MALDI-TOF MS was 84% for species (I
CONCLUSIONS
CONCLUSIONS
Our research showed that MALDI-TOF-MS was satisfactory in genus identification of clinical pathogenic anaerobic bacteria. However, this method still suffers from different drawbacks in precise identification of rare anaerobe and species levels of common anaerobic bacteria.
Identifiants
pubmed: 31699042
doi: 10.1186/s12879-019-4584-0
pii: 10.1186/s12879-019-4584-0
pmc: PMC6836477
doi:
Types de publication
Journal Article
Meta-Analysis
Langues
eng
Sous-ensembles de citation
IM
Pagination
941Subventions
Organisme : Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province
ID : 17KJB360014
Organisme : the National Natural Science Foundation of China
ID : 81871734
Organisme : Jiangsu Privincial Natural Science Foundation
ID : BK20151154
Organisme : Jiangsu Privincial Medical Talent
ID : ZDRCA2016053
Organisme : Six talent peaks project of Jiangsu Province
ID : WSN-135
Organisme : Advanced health talent of six-one project of Jiangsu Province
ID : LGY2016042
Organisme : the Natural Science Foundation of Jiangsu Province
ID : Grants No BK20150209
Organisme : the National Natural Science Foundation of China
ID : 81902040
Organisme : the National Natural Science Foundation of China
ID : 81471994
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