Surfaceome analysis of Australian epidemic Bordetella pertussis reveals potential vaccine antigens.
Antigens, Bacterial
/ immunology
Australia
/ epidemiology
Bacterial Outer Membrane Proteins
/ immunology
Bordetella pertussis
/ drug effects
Cell Survival
/ drug effects
Dose-Response Relationship, Drug
Epidemics
/ prevention & control
Humans
Pertussis Vaccine
/ administration & dosage
Proteomics
/ methods
Trypsin
/ pharmacology
Virulence Factors, Bordetella
/ immunology
Whooping Cough
/ epidemiology
Acellular vaccine
Bordetella pertussis
Comparative proteomics
Housekeeping proteins
Moonlighting proteins
Pathogen evolution
Surface shaving
Surfaceome
Vaccine candidates
Whole cell vaccine
Journal
Vaccine
ISSN: 1873-2518
Titre abrégé: Vaccine
Pays: Netherlands
ID NLM: 8406899
Informations de publication
Date de publication:
16 01 2020
16 01 2020
Historique:
received:
15
07
2019
revised:
18
10
2019
accepted:
22
10
2019
pubmed:
11
11
2019
medline:
13
2
2021
entrez:
10
11
2019
Statut:
ppublish
Résumé
Since acellular vaccines (ACV) were introduced in Australia, epidemic Bordetella pertussis strains changed from single nucleotide polymorphism (SNP) cluster II to SNP cluster I. Our previous proteomic analysis identified potential proteomic adaptations in the whole cell and secretome of SNP cluster I. Additionally, current ACVs were shown to be less efficacious against cluster I in mice models and there is a pressing need to discover new antigens to improve the ACV. One important source of novel antigens is the surfaceome. Therefore, in this study we established surface shaving in B. pertussis to compare the surfaceome of SNP cluster I (L1423) and II (L1191), and identify novel surface antigens for vaccine development. Surface shaving using 1 μg of trypsin for 5 min identified 126 proteins with the most abundant being virulence-associated and known outer membrane proteins. Cell viability counts showed minimal lysis from shaving. The proportion of immunogenic proteins was higher in the surfaceome than in the whole cell and secretome. Key differences in the surfaceome were identified between SNP cluster I and II, consistent with those identified in the whole cell proteome and secretome. These differences include unique transport proteins and decreased immunogenic proteins in L1423, and provides further evidence of proteomic adaptation in SNP cluster I. Finally, a comparison of proteins in each sub-proteome identified 22 common proteins. These included 11 virulence proteins (Prn, PtxA, FhaB, CyaA, TcfA, SphB1, Vag8, BrkA, BopD, Bsp22 and BipA) and 11 housekeeping proteins (TuF, CtpA, TsF, OmpH, GltA, SucC, SucD, FusA, GroEL, BP3330 and BP3561) which were immunogenic, essential and consistently expressed thus demonstrating their potential as future targets. This study established surface shaving in B. pertussis, confirmed key expression differences and identified unknown surface proteins which may be potential vaccine antigens.
Identifiants
pubmed: 31703933
pii: S0264-410X(19)31442-2
doi: 10.1016/j.vaccine.2019.10.062
pii:
doi:
Substances chimiques
Antigens, Bacterial
0
Bacterial Outer Membrane Proteins
0
Pertussis Vaccine
0
Virulence Factors, Bordetella
0
Trypsin
EC 3.4.21.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
539-548Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.