Tissue Expression of Melanoma-associated Antigen A6 and Clinical Characteristics of Gastric Cancer.
Adenocarcinoma
/ metabolism
Adenocarcinoma, Mucinous
/ metabolism
Adult
Aged
Aged, 80 and over
Antigens, Neoplasm
/ genetics
Biomarkers, Tumor
/ genetics
Carcinoma, Signet Ring Cell
/ metabolism
Case-Control Studies
Female
Follow-Up Studies
Gastrectomy
/ mortality
Humans
Liver Neoplasms
/ metabolism
Lymphatic Metastasis
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Proteins
/ genetics
Neoplasm Recurrence, Local
/ metabolism
Prognosis
Stomach Neoplasms
/ metabolism
Survival Rate
Gastric cancer
MAGE family member A6
biomarker
expression
immunohistochemistry
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
16
09
2019
revised:
26
09
2019
accepted:
01
10
2019
entrez:
10
11
2019
pubmed:
11
11
2019
medline:
26
11
2019
Statut:
ppublish
Résumé
Gastric cancer (GC) exhibits heterogeneous clinical and molecular features, requiring the development of new biomarkers to further understand this disease. Our transcriptomic analysis detected overexpression of melanoma-associated antigen A6 (MAGEA6) in metastatic GC, leading us to determine the clinical significance of MAGEA6 in GC. Fourteen GC cell lines and 230 pairs of surgically resected gastric tissues were subjected to mRNA expression analysis. Polymerase chain reaction array analysis was performed to identify coordinately expressed cancer-related genes, and immunohistochemistry (IHC) was used to detected MAGEA6 expression in situ. MAGEA6 mRNA levels were positively correlated with the expression of matrix metallopeptidase 9 mRNA. MAGEA6 mRNA levels were higher in GC tissues compared with those in normal adjacent tissues. Patients with high MAGEA6 expression had significantly worse prognosis. MAGEA6 protein levels in primary lesions predicted the likelihood of recurrence. Overexpression of MAGEA6 in GC tissues represents a promising biomarker for assessing the malignant phenotype of GC.
Sections du résumé
BACKGROUND
BACKGROUND
Gastric cancer (GC) exhibits heterogeneous clinical and molecular features, requiring the development of new biomarkers to further understand this disease. Our transcriptomic analysis detected overexpression of melanoma-associated antigen A6 (MAGEA6) in metastatic GC, leading us to determine the clinical significance of MAGEA6 in GC.
MATERIALS AND METHODS
METHODS
Fourteen GC cell lines and 230 pairs of surgically resected gastric tissues were subjected to mRNA expression analysis. Polymerase chain reaction array analysis was performed to identify coordinately expressed cancer-related genes, and immunohistochemistry (IHC) was used to detected MAGEA6 expression in situ.
RESULTS
RESULTS
MAGEA6 mRNA levels were positively correlated with the expression of matrix metallopeptidase 9 mRNA. MAGEA6 mRNA levels were higher in GC tissues compared with those in normal adjacent tissues. Patients with high MAGEA6 expression had significantly worse prognosis. MAGEA6 protein levels in primary lesions predicted the likelihood of recurrence.
CONCLUSION
CONCLUSIONS
Overexpression of MAGEA6 in GC tissues represents a promising biomarker for assessing the malignant phenotype of GC.
Identifiants
pubmed: 31704814
pii: 39/11/5903
doi: 10.21873/anticanres.13794
doi:
Substances chimiques
Antigens, Neoplasm
0
Biomarkers, Tumor
0
MAGEA6 protein, human
0
Neoplasm Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5903-5910Informations de copyright
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.