Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit.
Amino Acid Sequence
Binding Sites
DNA-Directed DNA Polymerase
/ chemistry
Models, Molecular
Nucleic Acid Conformation
Protein Binding
Protein Biosynthesis
Protein Conformation, alpha-Helical
Protein Conformation, beta-Strand
Protein Interaction Domains and Motifs
RNA Precursors
/ chemistry
RNA, Ribosomal
/ chemistry
Ribosome Subunits, Large, Eukaryotic
/ genetics
Ribosome Subunits, Small, Eukaryotic
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ chemistry
Sequence Alignment
Sequence Homology, Amino Acid
Thermodynamics
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
10 01 2020
10 01 2020
Historique:
accepted:
04
11
2019
revised:
29
10
2019
received:
24
05
2019
pubmed:
21
11
2019
medline:
18
3
2020
entrez:
21
11
2019
Statut:
ppublish
Résumé
More than 200 assembly factors (AFs) are required for the production of ribosomes in yeast. The stepwise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a hierarchical manner to ensure correct maturation of the pre-rRNAs and assembly of the ribosomal proteins. Although decades of research have provided a wealth of insights into the functions of many AFs, others remain poorly characterized. Pol5 was initially classified with B-type DNA polymerases, however, several lines of evidence indicate the involvement of this protein in ribosome assembly. Here, we show that depletion of Pol5 affects the processing of pre-rRNAs destined for the both the large and small subunits. Furthermore, we identify binding sites for Pol5 in the 5' external transcribed spacer and within domain III of the 25S rRNA sequence. Consistent with this, we reveal that Pol5 is required for recruitment of ribosomal proteins that form the polypeptide exit tunnel in the LSU and that depletion of Pol5 impairs the release of 5' ETS fragments from early pre-40S particles. The dual functions of Pol5 in 60S assembly and recycling of pre-40S AFs suggest that this factor could contribute to ensuring the stoichiometric production of ribosomal subunits.
Identifiants
pubmed: 31745560
pii: 5634039
doi: 10.1093/nar/gkz1079
pmc: PMC7145529
doi:
Substances chimiques
RNA Precursors
0
RNA, Ribosomal
0
Saccharomyces cerevisiae Proteins
0
RNA, ribosomal, 25S
130527-23-0
DNA-Directed DNA Polymerase
EC 2.7.7.7
POL5 protein, S cerevisiae
EC 2.7.7.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
405-420Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
RNA. 2019 Nov;25(11):1561-1575
pubmed: 31413149
PLoS One. 2013 Jul 10;8(7):e68412
pubmed: 23874617
FEMS Yeast Res. 2019 Jan 1;19(1):
pubmed: 30445532
Nat Struct Mol Biol. 2017 Nov;24(11):944-953
pubmed: 28945246
Genes Dev. 2015 Jul 1;29(13):1432-46
pubmed: 26159998
Nat Rev Mol Cell Biol. 2019 Feb;20(2):116-131
pubmed: 30467428
Cell. 2016 Jul 14;166(2):380-393
pubmed: 27419870
Methods Mol Biol. 1998;77:257-70
pubmed: 9770675
Nucleic Acids Res. 2017 May 5;45(8):4853-4865
pubmed: 28115637
PLoS One. 2012;7(3):e32552
pubmed: 22431976
Nat Struct Mol Biol. 2012 Aug;19(8):744-53
pubmed: 22751017
Mol Cell Biol. 2010 Mar;30(5):1130-44
pubmed: 20038530
J Biol Chem. 2012 Jul 13;287(29):24365-77
pubmed: 22645127
Nature. 2016 May 25;534(7605):133-7
pubmed: 27251291
Nucleic Acids Res. 2013 Nov;41(20):9461-70
pubmed: 23945946
Yeast. 1998 Jul;14(10):953-61
pubmed: 9717241
EMBO J. 2011 Sep 16;30(19):4020-32
pubmed: 21926967
Sci Rep. 2017 Jun 9;7(1):3169
pubmed: 28600509
EMBO J. 2011 Aug 02;30(19):4006-19
pubmed: 21811236
Nucleic Acids Res. 2013 Feb 1;41(3):1965-83
pubmed: 23268442
Mol Cell. 2010 Jun 11;38(5):712-21
pubmed: 20542003
Cell. 2012 Jul 6;150(1):111-21
pubmed: 22770215
PLoS Biol. 2017 Mar 10;15(3):e2000245
pubmed: 28282370
Nat Commun. 2016 Jun 29;7:12090
pubmed: 27354316
Nat Commun. 2014 Mar 24;5:3491
pubmed: 24662372
Mol Cell. 2013 Dec 12;52(5):707-19
pubmed: 24239293
Genes Dev. 2004 Oct 15;18(20):2506-17
pubmed: 15489292
EMBO Rep. 2008 Dec;9(12):1230-6
pubmed: 18833290
Elife. 2017 Feb 28;6:
pubmed: 28244370
J Mol Biol. 1998 Apr 24;278(1):67-78
pubmed: 9571034
Nucleic Acids Res. 2013 Sep;41(16):7889-904
pubmed: 23788678
Mol Cell. 2017 Sep 21;67(6):990-1000.e3
pubmed: 28890337
PLoS Genet. 2017 Mar 29;13(3):e1006699
pubmed: 28355211
EMBO J. 2002 Oct 15;21(20):5539-47
pubmed: 12374754
PLoS One. 2014 Feb 05;9(2):e88222
pubmed: 24505437
Biology (Basel). 2012 Dec 14;1(3):895-905
pubmed: 24832523
Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9133-8
pubmed: 12093911
Genes Dev. 2007 Oct 15;21(20):2580-92
pubmed: 17938242
Nat Struct Mol Biol. 2017 Sep 7;24(9):689-699
pubmed: 28880863
Mol Cell. 2009 Nov 25;36(4):583-92
pubmed: 19941819
J Mol Biol. 1972 Mar 28;65(2):227-42
pubmed: 4557192
Science. 2017 Jan 13;355(6321):
pubmed: 27980088
Science. 1996 Apr 12;272(5259):268-70
pubmed: 8602511
Mol Cell. 2015 Dec 3;60(5):808-815
pubmed: 26638174
Methods Enzymol. 2012;511:275-88
pubmed: 22713325
Nat Commun. 2018 Dec 19;9(1):5383
pubmed: 30568249
J Cell Biol. 2018 Jul 2;217(7):2503-2518
pubmed: 29691304
EMBO J. 1994 May 15;13(10):2452-63
pubmed: 7515008
Nucleic Acids Res. 2012 Sep 1;40(17):8646-61
pubmed: 22735702
Mol Cell. 2015 Sep 3;59(5):858-66
pubmed: 26340425
Nature. 2002 Jun 27;417(6892):967-70
pubmed: 12068309
Nat Commun. 2017 Nov 27;8(1):1787
pubmed: 29176610
Biochem Soc Trans. 2012 Aug;40(4):850-5
pubmed: 22817747
Mol Cell. 2004 Jan 30;13(2):225-39
pubmed: 14759368
Mol Cell Biol. 2007 Aug;27(15):5414-29
pubmed: 17515605
Mol Cell Biol. 2011 Oct;31(20):4156-64
pubmed: 21825077
Nat Struct Mol Biol. 2017 Nov;24(11):954-964
pubmed: 28967883
Nucleic Acids Res. 2013 Oct;41(18):8628-36
pubmed: 23880660
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
PLoS One. 2009 Dec 11;4(12):e8249
pubmed: 20011513
PLoS Biol. 2016 Jun 09;14(6):e1002480
pubmed: 27280440
J Mol Biol. 2000 Feb 11;296(1):7-17
pubmed: 10656814
PLoS One. 2017 Jul 7;12(7):e0179405
pubmed: 28686620
Cell. 2017 Dec 14;171(7):1599-1610.e14
pubmed: 29245012
Mol Cell Biol. 2012 Jan;32(2):430-44
pubmed: 22083961
Mol Cell. 2009 Dec 11;36(5):768-81
pubmed: 20005841
PLoS One. 2014 Dec 12;9(12):e114898
pubmed: 25501974
Front Microbiol. 2017 Feb 24;8:286
pubmed: 28286499
Genome Biol. 2014 Jan 07;15(1):R8
pubmed: 24393166
Nucleic Acids Res. 2001 Dec 15;29(24):5001-8
pubmed: 11812830
Nucleic Acids Res. 2013 Jan;41(2):1191-210
pubmed: 23209026
Annu Rev Genet. 1999;33:261-311
pubmed: 10690410
RNA. 1999 Jun;5(6):779-93
pubmed: 10376877
Cell Cycle. 2003 Mar-Apr;2(2):120-2
pubmed: 12695662
Mol Cell Biol. 2006 Oct;26(20):7437-50
pubmed: 16908538
Genes Dev. 2014 Jan 15;28(2):198-210
pubmed: 24449272
Nucleic Acids Res. 2015 Jan;43(1):553-64
pubmed: 25477391
Nature. 2018 Apr 5;556(7699):126-129
pubmed: 29512650
RNA. 2005 Oct;11(10):1571-8
pubmed: 16131592
Nucleic Acids Res. 2011 Oct;39(18):8105-21
pubmed: 21724601
Mol Cell. 2004 Dec 22;16(6):943-54
pubmed: 15610737
Mol Cell. 2010 Mar 26;37(6):809-20
pubmed: 20347423
Mol Cell. 2010 Jul 30;39(2):196-208
pubmed: 20670889
Nucleic Acids Res. 2009 Dec;37(22):7519-32
pubmed: 19789271
J Biol Chem. 2009 Dec 11;284(50):35079-91
pubmed: 19801658