Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs.


Journal

Journal of biomolecular NMR
ISSN: 1573-5001
Titre abrégé: J Biomol NMR
Pays: Netherlands
ID NLM: 9110829

Informations de publication

Date de publication:
Jan 2020
Historique:
received: 10 09 2019
accepted: 11 11 2019
pubmed: 23 11 2019
medline: 10 10 2020
entrez: 23 11 2019
Statut: ppublish

Résumé

Resonance assignments are challenging for membrane proteins due to the size of the lipid/detergent-protein complex and the presence of line-broadening from conformational exchange. As a consequence, many correlations are missing in the triple-resonance NMR experiments typically used for assignments. Herein, we present an approach in which correlations from these solution-state NMR experiments are supplemented by data from

Identifiants

pubmed: 31754899
doi: 10.1007/s10858-019-00289-7
pii: 10.1007/s10858-019-00289-7
pmc: PMC7015963
doi:

Substances chimiques

Bacteriorhodopsins 53026-44-1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

45-60

Subventions

Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
ID : Grant No 310030_159453
Organisme : Forschungskredit der Universität Zürich
ID : FK-18-083
Organisme : INEXT
ID : 653706

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Auteurs

Laurens Kooijman (L)

Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.

Philipp Ansorge (P)

Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.

Matthias Schuster (M)

Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.

Christian Baumann (C)

Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.

Frank Löhr (F)

Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Straße 9, 60438, Frankfurt am Main, Germany.

Simon Jurt (S)

Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.

Peter Güntert (P)

Institute of Biophysical Chemistry and Center for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Straße 9, 60438, Frankfurt am Main, Germany.
Laboratory of Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 1-5/10, 8093, Zurich, Switzerland.
Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.

Oliver Zerbe (O)

Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland. oliver.zerbe@chem.uzh.ch.

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Classifications MeSH