Metabolic transcriptional analysis on copper tolerance in moderate thermophilic bioleaching microorganism Acidithiobacillus caldus.
A. caldus
Copper stress
RNA-Seq
Transcriptome
qRT-PCR
Journal
Journal of industrial microbiology & biotechnology
ISSN: 1476-5535
Titre abrégé: J Ind Microbiol Biotechnol
Pays: Germany
ID NLM: 9705544
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
20
08
2019
accepted:
05
11
2019
pubmed:
24
11
2019
medline:
15
5
2020
entrez:
24
11
2019
Statut:
ppublish
Résumé
Bioleaching, an alternative environmental smelting technology, typically uses high concentrations of heavy metal ions, especially in the subsequent phase, due to metal ion accumulation from the mineral. In this study, we analyzed the overall response of the bioleaching microorganism Acidithiobacillus caldus to copper stress through physiological and transcriptomic analyses. Scanning electron microscopy results showed higher extracellular polymeric substances secretion and cell aggregation under copper stress. Intracellular levels of glutamic acid, glycine and cysteine increased, favoring the synthesis of glutathione for maintenance of the oxidation-reduction state. GSH, during copper stress conditions, the activity of GSH-PX and CAT increased, resulting in reduced oxidative damage while maintaining stable intracellular pH. Higher unsaturated and cyclopropane fatty acid levels resulted in increased membrane fluidity and compactness and decreased ATP levels to support the energy requirements for stress resistance. Initially, H
Identifiants
pubmed: 31758413
doi: 10.1007/s10295-019-02247-6
pii: 10.1007/s10295-019-02247-6
doi:
Substances chimiques
Sulfates
0
Copper
789U1901C5
Cysteine
K848JZ4886
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
21-33Subventions
Organisme : National Natural Science Foundation of China
ID : No. 21878128
Organisme : National Natural Science Foundation of China
ID : No. 21776113
Organisme : National Natural Science Foundation of China
ID : No. 21606110
Organisme : National Natural Science Foundation of China
ID : No. 31701582
Organisme : the Social Development Projects of Jiangsu Province
ID : BE2017625
Organisme : China Postdoctoral Science Foundation
ID : 2016M601716
Organisme : the funding of Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education
ID : KLCCB-KF201909
Organisme : the funding of Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education
ID : KLCCB-KF201907
Organisme : the funding of Key Laboratory of Industrial Biotechnology, Ministry of Education
ID : KLIB-KF201903
Organisme : the funding of Key Laboratory of Industrial Biotechnology, Ministry of Education
ID : KLIB-KF201906
Organisme : the Fundamental Research Funds for the Central Universities
ID : No. 2050205
Organisme : the Priority Academic Program Development of Jiangsu Higher Education Institutions, and Program of Introducing Talents of Discipline to Universities
ID : No. 111-2-06
Organisme : national first-class discipline program of Light Industry Technology and Engineering
ID : LITE2018-23
Références
Can J Microbiol. 1997 Jan;43(1):70-7
pubmed: 9057297
Toxicology. 2003 Jul 15;189(1-2):147-63
pubmed: 12821289
Appl Microbiol Biotechnol. 2017 May;101(10):3991-4008
pubmed: 28409384
J Ind Microbiol Biotechnol. 2009 Mar;36(3):461-5
pubmed: 19104861
J Proteomics. 2016 Aug 11;145:37-45
pubmed: 27079981
Nature. 1994 May 19;369(6477):242-5
pubmed: 8183345
BMC Microbiol. 2014 Jul 04;14:179
pubmed: 24993543
Biotechnol Adv. 2010 Nov-Dec;28(6):839-48
pubmed: 20627124
Int J Food Microbiol. 2016 Apr 2;222:16-22
pubmed: 26828814
Biochim Biophys Acta Mol Cell Res. 2017 Mar;1864(3):604-611
pubmed: 28063999
Environ Microbiol Rep. 2016 Oct;8(5):738-751
pubmed: 27337207
Extremophiles. 2015 Sep;19(5):1029-39
pubmed: 26264736
Mol Microbiol. 2003 Aug;49(3):807-21
pubmed: 12864861
Nat Chem Biol. 2017 Sep;13(9):1016-1021
pubmed: 28759019
Lipids. 2017 Aug;52(8):729-735
pubmed: 28660529
J Ind Microbiol Biotechnol. 2012 Jul;39(7):1031-9
pubmed: 22366811
J Neurochem. 1989 Feb;52(2):515-20
pubmed: 2911028
Appl Environ Microbiol. 2018 Jan 17;84(3):
pubmed: 29150517
J Biol Chem. 2008 Sep 12;283(37):25178-85
pubmed: 18650437
Front Microbiol. 2016 Dec 06;7:1960
pubmed: 27999570
Microbiology. 1994 Dec;140 ( Pt 12):3451-6
pubmed: 7533596
Bioresour Technol. 2015 Nov;196:694-706
pubmed: 26318845
J Bacteriol. 2000 Oct;182(20):5864-71
pubmed: 11004187
Plant Physiol. 2011 May;156(1):254-62
pubmed: 21427281
Appl Environ Microbiol. 1985 Mar;49(3):599-607
pubmed: 3994368
Appl Environ Microbiol. 2010 Jul;76(13):4185-93
pubmed: 20453144
PLoS One. 2012;7(9):e39470
pubmed: 22984393
J Biol Chem. 2000 Apr 14;275(15):10727-30
pubmed: 10753862
Biochemistry. 2017 Jun 20;56(24):3129-3141
pubmed: 28549213
Proc Natl Acad Sci U S A. 2009 May 19;106(20):8344-9
pubmed: 19416816
Genes (Basel). 2018 Jul 10;9(7):null
pubmed: 29996532
Cell Biol Toxicol. 1994 Feb;10(1):1-22
pubmed: 8076219
J Biol Chem. 2007 Dec 7;282(49):35695-702
pubmed: 17893146
J Ind Microbiol Biotechnol. 2017 Dec;44(12):1613-1625
pubmed: 28770421
Environ Technol. 2010 Jul-Aug;31(8-9):915-33
pubmed: 20662381
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
Indian J Med Res. 2008 Oct;128(4):501-23
pubmed: 19106443
Curr Opin Struct Biol. 2007 Aug;17(4):412-8
pubmed: 17723295
Front Microbiol. 2018 Jun 08;9:1255
pubmed: 29937764
Sci Rep. 2016 Dec 19;6:39065
pubmed: 27991525
FEMS Microbiol Lett. 2011 Oct;323(2):142-50
pubmed: 22092713
Annu Rev Microbiol. 1996;50:753-89
pubmed: 8905098
Chemosphere. 2018 Jul;203:76-82
pubmed: 29609104
Biotechnol Rep (Amst). 2016 Dec 23;13:58-71
pubmed: 28352564
Front Microbiol. 2011 Feb 10;2:17
pubmed: 21687411
Am J Clin Nutr. 1996 May;63(5):797S-811S
pubmed: 8615367
J Basic Microbiol. 2010 Dec;50(6):591-8
pubmed: 21072861
Appl Environ Microbiol. 2017 Mar 17;83(7):
pubmed: 28115381
J Biol Chem. 2011 Nov 4;286(44):38583-91
pubmed: 21900248
Front Plant Sci. 2014 Nov 21;5:662
pubmed: 25484889
Biol Trace Elem Res. 2013 Apr;152(1):91-7
pubmed: 23344975