Isolation and Genotyping of Toxoplasma gondii Strains.

Agglutination Tests Biological Assay Genetic Variation / genetics Genotype Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Toxoplasma / genetics
Genetic diversity Genotype Modified agglutination test (MAT) PCR RFLP Toxoplasma

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 24 11 2019
pubmed: 24 11 2019
medline: 12 1 2021
Statut: ppublish

Résumé

Toxoplasma gondii is a protozoan parasite that infects mammals and birds. Molecular epidemiology and population genetic studies have revealed widespread and distinct distribution of different T. gondii genotypes globally. Animals (domestic and wild) are the reservoirs for transmission of this parasite to humans. Recent development in molecular genotyping methods allowed us to identify parasite strains with high resolution and to dissect transmission patterns among different hosts. However, current data in the literature is still limited and fragmented. Here, we summarize a set of protocols that can be used to identify T. gondii infection in clinically normal animals, isolate the parasite by bioassay using animal tissues, extract parasite DNA from tissue samples, and finally identify the parasite by multilocus PCR-RFLP genotyping. We hope these protocols provide essential tools to study genetic diversity, population structure and transmission dynamics of T. gondii. Accumulation of the information will allow us to better understand, control, and prevent T. gondii infection in the future.

Identifiants

DOI: 10.1007/978-1-4939-9857-9_3 PMID: 31758446
pubmed: 31758446
doi: 10.1007/978-1-4939-9857-9_3
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

49-80

Auteurs

Chunlei Su (C)

Department of Microbiology, University of Tennessee, Knoxville, TN, USA. csu1@utk.edu.

Jitender P Dubey (JP)

Agricultural Research Service, Animal Parasitic Diseases Laboratory, United States Department of Agriculture (USDA), Beltsville Agricultural Research Center, Beltsville, MD, USA. jitender.dubey@ars.usda.gov.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Tests sérologiques › Tests d'agglutination › Isolation and Genotyping of Toxoplasma gondii Strains.
questionsmedicales.fr › Techniques d'investigation › Dosage biologique › Isolation and Genotyping of Toxoplasma gondii Strains.
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Isolation and Genotyping of Toxoplasma gondii Strains.
questionsmedicales.fr › Phénomènes génétiques › Génotype › Isolation and Genotyping of Toxoplasma gondii Strains.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Isolation and Genotyping of Toxoplasma gondii Strains.
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Polymorphisme génétique › Polymorphisme de restriction › Isolation and Genotyping of Toxoplasma gondii Strains.
questionsmedicales.fr › Eucaryotes › Alveolata › Apicomplexa › Coccidia › Eimeriida › Sarcocystidae › Toxoplasma › Isolation and Genotyping of Toxoplasma gondii Strains.