Molecular techniques and their limitations shape our view of the holobiont.

Environmental Microbiology Microbiota Symbiosis
Evolutionary inference Holobiont Imaging Metagenomic sequencing Model system Multi-omics

Journal

Zoology (Jena, Germany)
ISSN: 1873-2720
Titre abrégé: Zoology (Jena)
Pays: Germany
ID NLM: 9435608

Informations de publication

Date de publication:
12 2019
Historique:
received: 29 03 2019
revised: 08 07 2019
accepted: 12 07 2019
pubmed: 24 11 2019
medline: 17 6 2020
entrez: 24 11 2019
Statut: ppublish

Résumé

It is now recognised that the biology of almost any organism cannot be fully understood without recognising the existence and potential functional importance of associated microbes. Arguably, the emergence of this holistic viewpoint may never have occurred without the development of a crucial molecular technique, 16S rDNA amplicon sequencing, which allowed microbial communities to be easily profiled across a broad range of contexts. A diverse array of molecular techniques are now used to profile microbial communities, infer their evolutionary histories, visualise them in host tissues, and measure their molecular activity. In this review, we examine each of these categories of measurement and inference with a focus on the questions they make tractable, and the degree to which their capabilities and limitations shape our view of the holobiont.

Identifiants

DOI: 10.1016/j.zool.2019.125695 PMID: 31759226
pubmed: 31759226
pii: S0944-2006(19)30053-4
doi: 10.1016/j.zool.2019.125695
pii:
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

125695

Informations de copyright

Copyright © 2019 Elsevier GmbH. All rights reserved.

Auteurs

Ira Cooke (I)

Department of Molecular and Cell Biology, James Cook University, Townsville, QLD, 4811, Australia; Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, QLD, 4811, Australia. Electronic address: ira.cooke@jcu.edu.au.

Oliver Mead (O)

ARC Centre of Excellence for Coral Reef Studies, Australian National University, Canberra, ACT, 2601, Australia; Research School of Biology, Australian National University, Canberra, ACT, 2601, Australia.

Casey Whalen (C)

Department of Molecular and Cell Biology, James Cook University, Townsville, QLD, 4811, Australia; Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, QLD, 4811, Australia; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD, 4811, Australia.

Chloë Boote (C)

Department of Molecular and Cell Biology, James Cook University, Townsville, QLD, 4811, Australia; Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, QLD, 4811, Australia; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD, 4811, Australia.

Aurelie Moya (A)

Department of Molecular and Cell Biology, James Cook University, Townsville, QLD, 4811, Australia; Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, QLD, 4811, Australia; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD, 4811, Australia.

Hua Ying (H)

Research School of Biology, Australian National University, Canberra, ACT, 2601, Australia.

Steven Robbins (S)

Australian Center for Ecogenomics, University of Queensland, St. Lucia, QLD, 4072, Australia.

Jan M Strugnell (JM)

Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, QLD, 4811, Australia; Centre of Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, 4810, QLD, Australia; Department of Ecology, Environment and Evolution, School of Life Sciences, La Trobe University, Melbourne, 3083, Australia.

Aaron Darling (A)

The ithree institute, University of Technology Sydney, Ultimo, NSW, 2007, Australia.

David Miller (D)

Department of Molecular and Cell Biology, James Cook University, Townsville, QLD, 4811, Australia; Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, QLD, 4811, Australia; ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD, 4811, Australia.

Christian R Voolstra (CR)

Department of Biology, University of Konstanz, Konstanz, 78457, Germany.

Maja Adamska (M)

ARC Centre of Excellence for Coral Reef Studies, Australian National University, Canberra, ACT, 2601, Australia; Research School of Biology, Australian National University, Canberra, ACT, 2601, Australia.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Molecular techniques and their limitations...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Molecular techniques and their limitations...
questionsmedicales.fr › Phénomènes biologiques › Symbiose › Molecular techniques and their limitations...