DNA aneuploidy and centrosome amplification in canine tumor cell lines.

Aneuploidy Animals Cell Line, Tumor Centrosome / metabolism DNA, Neoplasm / genetics Dogs Neoplasms / genetics
Canine tumor cell line Centrosome amplification DNA aneuploidy

Journal

Tissue & cell
ISSN: 1532-3072
Titre abrégé: Tissue Cell
Pays: Scotland
ID NLM: 0214745

Informations de publication

Date de publication:
Dec 2019
Historique:
received: 10 07 2019
revised: 03 09 2019
accepted: 13 09 2019
entrez: 25 11 2019
pubmed: 25 11 2019
medline: 17 4 2020
Statut: ppublish

Résumé

DNA aneuploidy, the altered DNA content of a cell, is a common feature of canine tumors. However, it is unclear whether aneuploid DNA in canine tumor cells show centrosome amplification (CA), which contributes to numerical and structural chromosome aberrations that result in DNA aneuploidy. Here, we evaluated whether DNA aneuploidy and CA occur concurrently in canine tumor cell lines. Centrosome numbers were evaluated in 18 canine tumor cell lines by immunocytochemistry with anti-γ-tubulin antibody, and DNA content was evaluated by flow cytometry using propidium iodide. A total of 15 cell lines showed DNA aneuploidy, and CA was observed in 5 of these 15 cell lines. Together, our results suggest that DNA aneuploidy in canine tumor cells might be explained at least in part by CA. In addition, cell lines with CA may be useful tools to examine the detailed relationship between CA and DNA aneuploidy and the molecular mechanism of CA in canine tumor.

Identifiants

DOI: 10.1016/j.tice.2019.09.003 PMID: 31759409
pubmed: 31759409
pii: S0040-8166(19)30283-6
doi: 10.1016/j.tice.2019.09.003
pii:
doi:

Substances chimiques

DNA, Neoplasm 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

67-71

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Yoshifumi Endo (Y)

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Manabu Watanabe (M)

Laboratory of Functional Genomics, Department of Medical Genome Science, Graduate School of Frontier Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.

Nozomi Miyajima-Magara (N)

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Maki Igarashi (M)

Laboratory of Functional Genomics, Department of Medical Genome Science, Graduate School of Frontier Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan; Biochemistry Division, National Cancer Center Research Institute, 5-1-1, Tsukiji, Chuo, Tokyo 104-0045, Japan.

Manabu Mochizuki (M)

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Ryohei Nishimura (R)

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Sumio Sugano (S)

Laboratory of Functional Genomics, Department of Medical Genome Science, Graduate School of Frontier Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.

Nobuo Sasaki (N)

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Takayuki Nakagawa (T)

Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: anakaga@mail.ecc.u-tokyo.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Ploïdies › Aneuploïdie › DNA aneuploidy and centrosome amplification in...
questionsmedicales.fr › Eucaryotes › Animaux › DNA aneuploidy and centrosome amplification in...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › DNA aneuploidy and centrosome amplification in...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Appareil du fuseau › Pôles du fuseau › Centrosome › DNA aneuploidy and centrosome amplification in...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN tumoral › DNA aneuploidy and centrosome amplification in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Carnivora › Pinnipedia › Canidae › Chiens › DNA aneuploidy and centrosome amplification in...
questionsmedicales.fr › Tumeurs › DNA aneuploidy and centrosome amplification in...