Genome-wide histone modification profiling of inner cell mass and trophectoderm of bovine blastocysts by RAT-ChIP.
Animals
Blastocyst
/ cytology
Blastocyst Inner Cell Mass
/ metabolism
Cattle
Cell Line
Chromatin
/ chemistry
Chromatin Immunoprecipitation
Fertilization in Vitro
Genome
High-Throughput Nucleotide Sequencing
Histones
/ genetics
Humans
Oocytes
/ cytology
Protein Processing, Post-Translational
Sequence Analysis, DNA
Trophoblasts
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
06
2019
accepted:
12
11
2019
entrez:
26
11
2019
pubmed:
26
11
2019
medline:
24
3
2020
Statut:
epublish
Résumé
Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) has revolutionized our understanding of chromatin-related biological processes. The method, however, requires thousands of cells and has therefore limited applications in situations where cell numbers are limited. Here we describe a novel method called Restriction Assisted Tagmentation Chromatin Immunoprecipitation (RAT-ChIP) that enables global histone modification profiling from as few as 100 cells. The method is simple, cost-effective and takes a single day to complete. We demonstrate the sensitivity of the method by deriving the first genome-wide maps of histone H3K4me3 and H3K27me3 modifications of inner cell mass and trophectoderm of bovine blastocyst stage embryos.
Identifiants
pubmed: 31765427
doi: 10.1371/journal.pone.0225801
pii: PONE-D-19-18181
pmc: PMC6876874
doi:
Substances chimiques
Chromatin
0
Histones
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0225801Déclaration de conflit d'intérêts
The RAT-ChIP method is subject to a patent application (P1433PC00) by TO, AK and AS.
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