Adaptive evolution reveals a tradeoff between growth rate and oxidative stress during naphthoquinone-based aerobic respiration.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
10 12 2019
Historique:
pubmed: 27 11 2019
medline: 28 4 2020
entrez: 27 11 2019
Statut: ppublish

Résumé

Evolution fine-tunes biological pathways to achieve a robust cellular physiology. Two and a half billion years ago, rapidly rising levels of oxygen as a byproduct of blooming cyanobacterial photosynthesis resulted in a redox upshift in microbial energetics. The appearance of higher-redox-potential respiratory quinone, ubiquinone (UQ), is believed to be an adaptive response to this environmental transition. However, the majority of bacterial species are still dependent on the ancient respiratory quinone, naphthoquinone (NQ). Gammaproteobacteria can biosynthesize both of these respiratory quinones, where UQ has been associated with aerobic lifestyle and NQ with anaerobic lifestyle. We engineered an obligate NQ-dependent γ-proteobacterium,

Identifiants

pubmed: 31767748
pii: 1909987116
doi: 10.1073/pnas.1909987116
pmc: PMC6911176
doi:

Substances chimiques

Naphthoquinones 0
Reactive Oxygen Species 0
Oxo-Acid-Lyases EC 4.1.3.-
chorismate pyruvate lyase EC 4.1.3.-
Oxygen S88TT14065

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

25287-25292

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM057089
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI124316
Pays : United States

Informations de copyright

Copyright © 2019 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

Amitesh Anand (A)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Ke Chen (K)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Laurence Yang (L)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Anand V Sastry (AV)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Connor A Olson (CA)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Saugat Poudel (S)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Yara Seif (Y)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Ying Hefner (Y)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Patrick V Phaneuf (PV)

Bioinformatics and Systems Biology Program, University of California San Diego, La Jolla, CA 92093.

Sibei Xu (S)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Richard Szubin (R)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.

Adam M Feist (AM)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093.
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark.

Bernhard O Palsson (BO)

Department of Bioengineering, University of California San Diego, La Jolla, CA 92093; palsson@ucsd.edu.
Bioinformatics and Systems Biology Program, University of California San Diego, La Jolla, CA 92093.
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Kongens, Lyngby, Denmark.

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