A diarylamine derived from anthranilic acid inhibits ZIKV replication.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 11 2019
27 11 2019
Historique:
received:
25
01
2019
accepted:
29
10
2019
entrez:
29
11
2019
pubmed:
30
11
2019
medline:
4
11
2020
Statut:
epublish
Résumé
Zika virus (ZIKV) is a mosquito-transmitted Flavivirus, originally identified in Uganda in 1947 and recently associated with a large outbreak in South America. Despite extensive efforts there are currently no approved antiviral compounds for treatment of ZIKV infection. Here we describe the antiviral activity of diarylamines derived from anthranilic acid (FAMs) against ZIKV. A synthetic FAM (E3) demonstrated anti-ZIKV potential by reducing viral replication up to 86%. We analyzed the possible mechanisms of action of FAM E3 by evaluating the intercalation of this compound into the viral dsRNA and its interaction with the RNA polymerase of bacteriophage SP6. However, FAM E3 did not act by these mechanisms. In silico results predicted that FAM E3 might bind to the ZIKV NS3 helicase suggesting that this protein could be one possible target of this compound. To test this, the thermal stability and the ATPase activity of the ZIKV NS3 helicase domain (NS3
Identifiants
pubmed: 31776405
doi: 10.1038/s41598-019-54169-z
pii: 10.1038/s41598-019-54169-z
pmc: PMC6881388
doi:
Substances chimiques
Amines
0
Antiviral Agents
0
Viral Proteins
0
ortho-Aminobenzoates
0
anthranilic acid
0YS975XI6W
NS3 protein, zika virus
EC 3.4.-
Serine Endopeptidases
EC 3.4.21.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17703Subventions
Organisme : Wellcome Trust
ID : 096670
Pays : United Kingdom
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